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508741 MilliporeDAPI Stain - CAS 28718-90-3 - Calbiochem

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl in H₂O.

DAPI Stain - CAS 28718-90-3 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
参考
Data Sheet

同义词: 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O

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概述

Replacement Information

重要规格表

CAS #	Empirical Formula
28718-90-3	C₁₆H₁₅N₅ • 2HCl

价格及供货情况

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Description
Overview	Cells are typically stained at 1 µg/ml.
Catalogue Number	508741
Brand Family	Calbiochem®
Synonyms	4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O

References
References	Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13. Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251. Hotz, M.A., et al. 1994. Cytometry 15, 237. Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249. Miller, T., et al. 1993. Biotechniques 15, 1042. Gray, J.W., et al. 1982. Science 238, 323. Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315. Russels, W.C., et al. 1975. Nature 253, 461.

References

Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.

Product Information
CAS number	28718-90-3
Form	Liquid
Formulation	A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
Hill Formula	C₁₆H₁₅N₅ • 2HCl
Chemical formula	C₁₆H₁₅N₅ • 2HCl
Reversible	Y
Structure formula Image
Quality Level	MQ200

Applications

Biological Information
Primary Target	DNA
Purity	≥97% by HPLC

Physicochemical Information
Cell permeable	Y
Emission max.
Excitation max.

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Protect from Light	Protect from light
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
5.08741.0001	04055977261370

Documentation

DAPI Stain - CAS 28718-90-3 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

参考

参考信息概述
Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13. Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251. Hotz, M.A., et al. 1994. Cytometry 15, 237. Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249. Miller, T., et al. 1993. Biotechniques 15, 1042. Gray, J.W., et al. 1982. Science 238, 323. Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315. Russels, W.C., et al. 1975. Nature 253, 461.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	28-March-2014 JSW
Synonyms	4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
Description	Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Form	Liquid
Formulation	A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
Intert gas (Yes/No)	Packaged under inert gas
CAS number	28718-90-3
Chemical formula	C₁₆H₁₅N₅ • 2HCl
Structure formula
Purity	≥97% by HPLC
Storage	Protect from light Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
Toxicity	Standard Handling
References	Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13. Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251. Hotz, M.A., et al. 1994. Cytometry 15, 237. Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249. Miller, T., et al. 1993. Biotechniques 15, 1042. Gray, J.W., et al. 1982. Science 238, 323. Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315. Russels, W.C., et al. 1975. Nature 253, 461.

种类

Life Science Research > Inhibitors and Biochemicals > Biochemicals > Dyes, Stains and Probes > DNA-Binding Dyes

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