Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
D-Tube™ Dialyzers Mini, Midi, Maxi, and Mega
The D-Tube™ Dialyzers be used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuge, or laborious steps to manipulate small sample volumes. The sample is added and removed using a standard laboratory pipet. Available with molecular weight cutoffs (MWCO) from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in four volume capacities: mini (10-250 µl), midi (50-800 micro;l), maxi (100-3000 µl), and mega (10ml, 15ml, and 20 ml). The membrane is ultra-clean, EDTA-treated regenerated cellulose, sulfur- and heavy metal-free.
The D-Tube Dialyzers Mini, Midi, and Maxi contain 10 D-Tube Dialyzers and one floating rack that can hold up to four D-Tube Dialyzers. The D-Tube Dialyzers Mega contain 10 or 50 tubes and one or 5 floating racks to hold one D-Tube Mega in the exchange buffer.
D-Tube™ Electroelution Accessory Kit
The D-Tube Electroelution Accessory Kit provides a support tray for the D-Tube Dialyzers (compatible with most commercially-available horizontal electrophoresis units) and optimized reagents for protein and nucleic acid precipitation following electroelution. The kit also contains an optimized buffer for the removal of SDS from extracted protein samples, making them compatible with MALDI-MS analysis.
The combination of D-Tube Dialyzers and D-Tube Electroelution Accessory Kit provides a unique tool for extraction of any protein, protein-protein and protein-DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels with 60% recovery yield in less than 2 hours. The procedure efficiently
recovers proteins and simultaneously removes ampholytes from proteins run on 2D gels. Extracted proteins are compatible with most downstream applications such as MALDIMS, animal immunization for antibody
production, HPLC analysis, peptide mapping, and functional assays. In addition, D-Tube Dialyzers can be used for oligonucleotides, RNA and DNA extraction from both polyacrylamide and agarose gels. Efficient
extraction (>90%) is achieved for 15 nt oligos and for up to 80 kbp DNA fragments.
Catalogue Number
71508
Brand Family
Novagen®
Features and benefits
Easy-to-handle dialyzers for buffer exchange and removal of urea, detergents
Ideal for electroelution of proteins, protein-DNA complexes, oligonucleotides, DNA, and RNA from polyacrylamide and agarose gels
One-step procedure that does not require syringes or any special equipment
