Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
An antifungal antibiotic that inhibits protein synthesis in eukaryotes but not prokaryotes. Competitively inhibits hFKBP12 (Ki = 3.4 µM). Triggers apoptosis in HL-60 cells, T cell hybridomas, Burkitt’s lymphoma cells, and other cell types. A 100 mg/ml solution of Cycloheximide (Cat. No. 239765) in DMSO (sterile-filtered) is also available.
Catalogue Number
239763
Brand Family
Calbiochem®
References
References
Christner, C., et al. 1999. J. Med. Chem.42, 3615. Lu, Q. and Mellgreen,R.L. 1996. Arch. Biochem. Biophys.334, 175. Chow, S.C., et al. 1995. Exp. Cell Res.216, 149. Waring, P. 1990. J. Biol. Chem.265, 14476. Obrig, T.G., et al. 1971. J. Biol. Chem.246, 174. Pestka, S. 1971. Annu. Rev. Microbiol.25, 487.
Very toxic if swallowed. Limited evidence of a carcinogenic effect. Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. May cause harm to the unborn child.
S Phrase
S: 45-53-61
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). Avoid exposure - obtain special instructions before use. Avoid release to the environment. Refer to special instructions/safety data sheet.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Highly Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+15°C to +30°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C, pH 3-5.
Hui Zeng, et al. (2005) Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways. American Journal of Physiology Gastrointestinal and Liver Physiologyin press,.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
01-March-2019 JSW
Description
An antifungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFKBP12 (Ki = 3.4 µM). Triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt's lymphoma cells, and a variety of other cell types, including rodent macrophages. Induces DNA fragmentation in macrophages, however it inhibits DNA cleavage in rat thymocytes treated with thapsigargin, methylprednisolone, and ionomycin. Rapidly destroyed in alkaline solutions.
Form
Off-white solid
CAS number
66-81-9
RTECS
MA4375000
Chemical formula
C₁₅H₂₃NO₄
Structure formula
Purity
≥90% by assay
Solubility
Chloroform, Ethanol, Methanol, or H₂O (10 mg/ml)
Storage
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C, pH 3-5.
Toxicity
Highly Toxic & Carcinogenic / Teratogenic
Merck USA index
14, 2728
References
Christner, C., et al. 1999. J. Med. Chem.42, 3615. Lu, Q. and Mellgreen,R.L. 1996. Arch. Biochem. Biophys.334, 175. Chow, S.C., et al. 1995. Exp. Cell Res.216, 149. Waring, P. 1990. J. Biol. Chem.265, 14476. Obrig, T.G., et al. 1971. J. Biol. Chem.246, 174. Pestka, S. 1971. Annu. Rev. Microbiol.25, 487.
Citation
Hui Zeng, et al. (2005) Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways. American Journal of Physiology Gastrointestinal and Liver Physiologyin press,.