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Human serum depleted of complement component C9 by affinity chromatography.
Catalogue Number
234409
Brand Family
Calbiochem®
References
Product Information
Unit of Definition
<b>Classical Pathway Activity:</b> One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.
<b>CH50 Functional Activity upon Reconstitution:</b> One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.
<b>Alternative Pathway Activity:</b> One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Form
Liquid
Formulation
In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.
Preservative
None
Applications
Biological Information
Source
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-June-2008 RFH
Description
Human serum depleted of complement component C9 by affinity chromatography. C9-depleted serum, sterile-filtered through a 0.22 µm filter. No C9 detected by Ouchterlony using various dilutions of goat anti-human C9 antibody.
Form
Liquid
Formulation
In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Classical Pathway Activity
Recommended volume C9-Dpl serum per assay: ≤25 µl
C9H50 units/mg purified C9 at the recommended input of C9-Dpl: ≥100,000 C9H50 units/mg
Functional Activity Ratio: ≥0.60 (C9H50/mg C9 : C9H50/mg C9 in NHS Std.)
CH50 Functional Activity upon Reconstitution
Reconstitution Ratio: ≥0.60 (CH50/ml C9-Dpl + C9 : CH50/ml NHS Std)
Alternative Pathway Activity
Input of NHS Standard to yield 1 APH50: ≤20 µl
Source
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Unit definition
Classical Pathway Activity: One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.
CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.
Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Preservative
None
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
