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Human serum that has been depleted of complement component C3.
Catalogue Number
234403
Brand Family
Calbiochem®
References
Product Information
Unit of Definition
<b>Classical Pathway Activity:</b> One C3H50 unit measured by classical pathway activation is defined as the amount of C3 required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C3-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.
Form
Clear, straw colored liquid
Formulation
In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2.
Applications
Biological Information
Source
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-June-2008 RFH
Description
Human serum that has been depleted of complement component C3.
Form
Clear, straw colored liquid
Formulation
In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Classical Pathway Activity
Recommended volume C3-Dpl serum per assay: ≤30 µl
C3H50 units/mg purified C3 at the recommended input of C3-Dpl: ≥2000 units/mg
Ratio: ≥0.60 (C3H50/mg C3)/(C3H50/mg C3 in NHS Std)
C3D Reconstituted With C3
Ratio: ≥0.6 (CH50/ml C3-Dpl + C3)/(CH50/ml NHS Std)
Source
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Unit definition
Classical Pathway Activity: One C3H50 unit measured by classical pathway activation is defined as the amount of C3 required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C3-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
