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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

234401 Sigma-AldrichComplement C1q-Depleted Serum, Human

Complement C1q-Depleted Serum, Human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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分析证书
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Replacement Information

价格及供货情况

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Description
Overview	Human serum that has been depleted of C1q complement component.
Catalogue Number	234401
Brand Family	Calbiochem®

References

Product Information
Unit of Definition	<b>Classical Pathway Activity:</b> One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. <b>CH50 Functional Activity upon Reconstitution:</b> One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. <b>Alternative Pathway Activity:</b> One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Form	Clear, amber solution
Formulation	In PBS, ≤0.1% sodium azide, pH 7.4.
Preservative	None
Quality Level	MQ100

Applications
Application Comments	No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis: CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++. • Recommended volume of C1q-Dpl serum per assay • C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl • Input of purified C1q to yield 1 C1qH50: • C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl • Input of C1q in NHS Complement standard to yield 1 C1qH50 • Ratio • Background A412 EA blank reading at the recommended input of C1q-Dpl CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++. • CH50/ml NHS Standard • CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml: • Ratio ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM. • APH50/ml NHS Standard • Input of NHS Standard to yield 1 APH50 • APH50/ml C1q-Dpl • Input of C1q-Dpl to yield 1 APH50 • AP Ratio

Applications

Application Comments

No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:

CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
• Recommended volume of C1q-Dpl serum per assay
• C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
• Input of purified C1q to yield 1 C1qH50:
• C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
• Input of C1q in NHS Complement standard to yield 1 C1qH50
• Ratio
• Background A412 EA blank reading at the recommended input of C1q-Dpl

CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
• CH50/ml NHS Standard
• CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
• Ratio

ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
• APH50/ml NHS Standard
• Input of NHS Standard to yield 1 APH50
• APH50/ml C1q-Dpl
• Input of C1q-Dpl to yield 1 APH50
• AP Ratio

Biological Information
Source	Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information
Contaminants	C1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
234401-1MLCN	04055977200195

Documentation

Complement C1q-Depleted Serum, Human MSDS

职位
物料安全数据表 (MSDS)

Complement C1q-Depleted Serum, Human 分析证书

标题	批号
234401	输入批号

引用

标题

Paramita Baruah, et al. (2006) The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells. Journal of Leukocyte Biology in press,.

Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	09-June-2008 RFH
Description	Human serum that has been depleted of C1q complement component. C1q-depleted human serum, sterile filtered through a 0.22 µm filter. This product has the following specifications:
Form	Clear, amber solution
Formulation	In PBS, ≤0.1% sodium azide, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Recommended reaction conditions	Classical Pathway Activity Recommended volume C1q-Dpl serum per assay: ≤ 40 µl C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl: ≥ 12,500 C1qH50/mg C1q input/C1qH50: ≤80 ng C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl: ≥3,500 units/ml Input of C1q in NHS Complement standard to yield 1 C1qH50: ≤ 20 ng C1q in NHS Standard Ratio: ≥ 0.20 (C1qH50/mg C1q)/(C1qH50/mg C1q in NHS Std) Background A412 EA blank reading at the recommended input of C1q-Dpl: ≤ 0.250 CH50 Functional Activity upon Reconstitution CH50/ml NHS Standard: ≥75 CH50 units/ml CH50/ml C1q-Dpl reconstituted with 100 µg C1q/ml: ≥40 CH50 units/ml Ratio: ≥0.50 (CH50/ml C1q-Dpl + C1q)/(CH50/ml NHS Std) Alternative Pathway Activity APH50 units/ml NHS Complement Standard: ≥50 APH50 units/ml Input of NHS Standard to yield 1 APH50: ≤20 µl APH50 units/ml C1q-Dpl: ≥50 APH50 units/ml Input of C1q-Dpl to yield 1 APH50: ≤20 µl AP Ratio: ≥0.50 (APH50/ml C1q-Dpl)/(APH50/ml NHS Std)
Source	Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Contaminants	C1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
Unit definition	Classical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Preservative	None
Comments	No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis: CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++. • Recommended volume of C1q-Dpl serum per assay • C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl • Input of purified C1q to yield 1 C1qH50: • C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl • Input of C1q in NHS Complement standard to yield 1 C1qH50 • Ratio • Background A412 EA blank reading at the recommended input of C1q-Dpl CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++. • CH50/ml NHS Standard • CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml: • Ratio ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM. • APH50/ml NHS Standard • Input of NHS Standard to yield 1 APH50 • APH50/ml C1q-Dpl • Input of C1q-Dpl to yield 1 APH50 • AP Ratio
Storage	Avoid freeze/thaw ≤ -70°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).
Toxicity	Standard Handling
Citation	Paramita Baruah, et al. (2006) The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells. Journal of Leukocyte Biology in press,. Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.

种类

Life Science Research > Proteins and Enzymes > Other Proteins

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