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Highly purified native, type IV collagen from human placenta. Useful as a reference standard for collagen Type IV. Subunit composition: a mixture of α1(IV)2α2(IV), α1(IV)3, and α2(IV)3. Typically used as a thin coating on tissue culture surfaces.
M.W. 450,000 (heterotrimer subunit).
Catalogue Number
234154
Brand Family
Calbiochem®
References
References
Miller, E.J., and Gay, S. 1987. Methods Enzymol. 144, 3. Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397. Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.
Miller, E.J., and Gay, S. 1987. Methods Enzymol. 144, 3. Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397. Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-February-2011 JSW
Description
Highly purified native, type IV collagen from human placenta. Aseptically filled. Highly purified preparation. Useful as a reference standard for collagen Type IV. Subunit composition: a mixture of α1(IV)2, α2(IV), α(IV)3, and α2(IV)3. Typically used as a thin coating on tissue culture surfaces.
Form
Liquid
Formulation
In sterile 2 mM HCl.
Concentration Label
Please refer to vial label for lot-specific concentration
Source
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number
9007-34-5
Purity
≥90% by SDS-PAGE
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
Merck USA index
14, 2480
References
Miller, E.J., and Gay, S. 1987. Methods Enzymol. 144, 3. Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397. Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.