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Highly purified, native, type I collagen from human placenta. Useful as a gel or thin coating for the attachment of cells. Recommended concentration: 0.2 - 2 µg/cm2 of growth surface.
Catalogue Number
234149
Brand Family
Calbiochem®
References
References
Bosco, M., et al. 1993. J. Cell Biol.120, 537. Leavesley, D.I., et al. 1993. J. Cell Biol.121, 163. Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys.301, 103. Streuli, C.H., et al. 1993. J. Cell Biol.120, 253. Karsenty, G., et al. 1991. J. Biol. Chem.266, 24842. Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Bosco, M., et al. 1993. J. Cell Biol.120, 537. Leavesley, D.I., et al. 1993. J. Cell Biol.121, 163. Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys.301, 103. Streuli, C.H., et al. 1993. J. Cell Biol.120, 253. Karsenty, G., et al. 1991. J. Biol. Chem.266, 24842. Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
引用
标题
Emily K. Thompson, et al. (2005) Endo180 binds to the C-terminal region of type I collagen. Journal of Biological Chemistry280, 22596-22605.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
18-June-2010 JSW
Description
Highly purified, native, type I collagen from human placenta. Useful as a gel or thin coating for the attachment of cells. Facilitates in vitro cultivation of cells and enhances cell-specific morphology and function. The recommended concentration is 0.2-2 µg/cm2 of growth surface.
Form
Liquid
Formulation
In 10 mM HCl.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Coating Procedure Guidelines Note: The optimal concentration for cell attachment and culture may differ for different cell types, and experimentation may be required to determine the optimal conditions for your cell culture system.
Human Collagen, Type I is typically used as a thin coating on plastic tissue culture plates. Alternatively, it may also be gelled. Guidelines for Coating Tissue Culture Plates (Thin Coating)
1. Add sufficient volume of collagen to provide desired coating concentration. A coating concentration of 0.2 µg/cm2 to 2 µg/cm2 is recommended, depending on the cell type. It is important that the volume added to the dish is sufficient to cover the growth surface. If necessary, dilute the collagen stock with 2 mM HCl.
2. Cover the growth surface completely covered and incubate for 2 h at room temperature.
3. Tilt the dish just enough to allow excess collagen to drain to the lowest point in dish. Remove excess material with sterile pipette.
4. Air dry the plate by leaving the lid slightly ajar in a laminar flow tissue culture hood or dry with a gentle stream of sterile gas.
5. Once dry, the plates are ready for use. Guidelines for Gelling
1. Dilute the collagen to desired concentration using 2 mM HCl.
2. Mix 9 parts Collagen, Type I, Human Placenta and 1 part 10X buffer or 10X media.
3. Add the mixture to the desired tissue culture vessel.
4. Incubate for 15 to 60 min at 37°C. Gel is then ready for use, but must be handled carefully.
Source
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number
9007-34-5
Purity
≥90% by SDS-PAGE
Solubility
Further dilute with acetic acid (10 mM)
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
Merck USA index
14, 2480
References
Bosco, M., et al. 1993. J. Cell Biol.120, 537. Leavesley, D.I., et al. 1993. J. Cell Biol.121, 163. Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys.301, 103. Streuli, C.H., et al. 1993. J. Cell Biol.120, 253. Karsenty, G., et al. 1991. J. Biol. Chem.266, 24842. Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Citation
Emily K. Thompson, et al. (2005) Endo180 binds to the C-terminal region of type I collagen. Journal of Biological Chemistry280, 22596-22605.
