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220477 Sigma-AldrichChitosanase, Streptomyces sp. N174

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

Chitosanase, Streptomyces sp. N174 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Chitosan N-acetylglucosaminohydrolase

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概述

Replacement Information

价格及供货情况

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Description
Overview	Native Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.
Catalogue Number	220477
Brand Family	Calbiochem®
Synonyms	Chitosan N-acetylglucosaminohydrolase

References
References	Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077. Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479. Fukamizo, T., et al. 1995. Biochem J. 311, 377. Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599. Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183. Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

Product Information
CAS number	51570-20-8
Unit of Definition	One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
EC number	3.2.1.132
Form	Liquid
Formulation	In 50 M sodium acetate, 50% glycerol, pH 5.0.
PI	7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C
Quality Level	MQ100

Applications

Biological Information
Specific Activity	≥25 units/mg protein

Physicochemical Information
Contaminants	chitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
220477-10UCN	04055977201123

Documentation

Chitosanase, Streptomyces sp. N174 MSDS

职位
物料安全数据表 (MSDS)

Chitosanase, Streptomyces sp. N174 分析证书

标题	批号
220477	输入批号

参考

参考信息概述
Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077. Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479. Fukamizo, T., et al. 1995. Biochem J. 311, 377. Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599. Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183. Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	07-March-2019 JSW
Synonyms	Chitosan N-acetylglucosaminohydrolase
Description	Native Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.
Form	Liquid
Formulation	In 50 M sodium acetate, 50% glycerol, pH 5.0.
CAS number	51570-20-8
EC number	3.2.1.132
Contaminants	chitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)
Specific activity	≥25 units/mg protein
Unit definition	One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077. Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479. Fukamizo, T., et al. 1995. Biochem J. 311, 377. Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599. Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183. Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

种类

Life Science Research > Proteins and Enzymes > Other Enzymes

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