Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
SpeedScreen: label-free liquid chromatography-mass spectrometry-based high-throughput screening for the discovery of orphan protein ligands I. Muckenschnabel, R. Falchetto, L.M. Mayr, and I. Filipuzzi Analytical Biochemistry, 324 (2004) 241-249
2004
Protein Concentration, Protein Desalting
问与答
问题
回答
What is the best way to load dry resin into a MultiScreen plate?
The best way to load dry resin into MultiScreen plates is to use a MultiScreen column loader. With this device, all 96 wells can be loaded simultaneously in minutes. The column loaders come in four sizes, 25ul 45ul, 80ul, and 100ul, optimized for a variety of key applications.
What column loader should I use for my application?
The column loader you chose will depend on how much resin you need to load and how much the beads swell when hydrated. Some of the more common resins are listed below with the appropriate column loader.
COLUMN LOADER
RESIN
APPLICATION
25ul
C18, C4, SCX
Sample prep for analytical instrumentation
45ul
Sephadex® G-50, G-75
Protein/DNA purification Dye terminator removal (standard and Big dyes)
