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An epoxysuccinyl peptide highly selective for rat liver cathepsin L (IC50 = 1.9 nM), while exhibiting little to no effect on rat liver cathepsin B at concentrations higher than 1000-5000 nM, in vitro. During autophagy, it has been shown through immunoblotting that CAA0225 (10 µg/ml) effectively inhibits the degradation of LC3-II and GABARAP compared to control, in nutrient-deprived HeLa and Huh-7 cells.
Catalogue Number
219502
Brand Family
Calbiochem®
References
References
Takahashi, K., et al. 2009. Biol. Pharm. Bull.32, 475. Ueno, T., et al. 2009. Autophagy5, 878.
Takahashi, K., et al. 2009. Biol. Pharm. Bull.32, 475. Ueno, T., et al. 2009. Autophagy5, 878.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-March-2011 RFH
Description
An epoxysuccinyl peptide highly selective for rat liver cathepsin L (IC50 = 1.9 nM), while exhibiting little to no effect on rat liver cathepsin B at concentrations higher than 1000-5000 nM, in vitro. During autophagy, it has been shown through immunoblotting that CAA0225 (10 µg/ml) effectively inhibits the degradation of LC3-II and GABARAP compared to control, in nutrient-deprived HeLa and Huh-7 cells.
Form
White solid
Chemical formula
C₂₈H₂₉N₃O₅
Structure formula
Purity
≥ 95% by HPLC
Solubility
DMSO (100 mg/ml)
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Takahashi, K., et al. 2009. Biol. Pharm. Bull.32, 475. Ueno, T., et al. 2009. Autophagy5, 878.
