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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

219360 Sigma-AldrichCathepsin D/E Substrate, Fluorogenic - Calbiochem

Cathepsin D/E Substrate, Fluorogenic, is an internally quenched fluorogenic 11-mer peptide that acts as a selective substrate for cathepsins D and E.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
Data Sheet

同义词: MOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂

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概述

Replacement Information

价格及供货情况

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Description
Overview	An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (k_cat/K_m = 15.6 µM^-1s^-1 and 10.9 µM^-1s^-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
Catalogue Number	219360
Brand Family	Calbiochem®
Synonyms	MOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂

References
References	Yasuda, Y., et al. 1999. J. Biochem. 126, 260. Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.

Product Information
ATP Competitive	N
Form	Yellow lyophilized solid
Formulation	Supplied as a trifluoroacetate salt.
Hill Formula	C₈₅H₁₂₂N₂₂O₁₉
Chemical formula	C₈₅H₁₂₂N₂₂O₁₉
Hygroscopic	Hygroscopic
Reversible	N
Quality Level	MQ100

Applications
Application	Cathepsin D/E Substrate, Fluorogenic, is an internally quenched fluorogenic 11-mer peptide that acts as a selective substrate for cathepsins D and E.

Biological Information
Primary Target	Substrate for cathepsins D and E
Primary Target IC<sub>50</sub>	kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively
Purity	≥97% by HPLC

Physicochemical Information
Cell permeable	N
Emission max.
Excitation max.
Peptide Sequence	MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂ MOCAc = (7-Methoxycoumarin-4-yl)acetyl

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Protect from Light	Protect from light
Hygroscopic	Hygroscopic
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
219360-1MGCN	04055977202182

Documentation

Cathepsin D/E Substrate, Fluorogenic - Calbiochem 分析证书

标题	批号
219360	输入批号

参考

参考信息概述
Yasuda, Y., et al. 1999. J. Biochem. 126, 260. Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	05-June-2008 RFH
Synonyms	MOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
Description	An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (k_cat/K_m = 15.6 µM^-1s^-1 and 10.9 µM^-1s^-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.
Form	Yellow lyophilized solid
Formulation	Supplied as a trifluoroacetate salt.
Intert gas (Yes/No)	Packaged under inert gas
Recommended reaction conditions	80 µl of 50 mM sodium acetate buffer, pH 4.0, 10 µl of 200 µM substrate solution, 10 µl of sample, incubate at 40°C for 10 min; terminate reaction with 2 ml of 5% TCA.
Chemical formula	C₈₅H₁₂₂N₂₂O₁₉
Peptide Sequence	MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂ MOCAc = (7-Methoxycoumarin-4-yl)acetyl
Purity	≥97% by HPLC
Solubility	DMSO (5 mg/ml)
Storage	Protect from light +2°C to +8°C Hygroscopic
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Standard Handling
References	Yasuda, Y., et al. 1999. J. Biochem. 126, 260. Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.

种类

Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Protease Substrates

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