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A fluorogenic substrate for caspase-6 (Mch2). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
Catalogue Number
218763
Brand Family
Calbiochem®
Synonyms
Z-VEID-AFC
References
References
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
04-June-2008 RFH
Synonyms
Z-VEID-AFC
Description
A fluorogenic substrate for caspase-6 (Mch2). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).
Form
Lyophilized
CAS number
219138-06-4
Chemical formula
C₃₈H₄₄F₃N₅O₁₂
Peptide Sequence
Z-Val-Glu-Ile-Asp-AFC
Purity
≥95% by HPLC
Solubility
DMSO (5 mg/ml)
Storage
Protect from moisture
Protect from light
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.