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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

208771 Sigma-AldrichCalpain Substrate III, Fluorogenic - Calbiochem

A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: (DABCYL)-TPLK~SPPPSPR-(EDANS)

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概述

Replacement Information

价格及供货情况

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Description
Overview	A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (k_cat/K_mx10^-2 (M^-1s^-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
Catalogue Number	208771
Brand Family	Calbiochem®
Synonyms	(DABCYL)-TPLK~SPPPSPR-(EDANS)

References
References	Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775.

Product Information
ATP Competitive	N
Form	Red lyophilized solid
Formulation	Supplied as a trifluoroacetate salt.
Hill Formula	C₈₀H₁₁₄N₂₀O₁₈S
Chemical formula	C₈₀H₁₁₄N₂₀O₁₈S
Reversible	N
Quality Level	MQ100

Applications

Biological Information
Primary Target	Substrate sequence that is optimized for calpain-1 and -2
Primary Target IC<sub>50</sub>	kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
Purity	≥98% by HPLC

Physicochemical Information
Cell permeable	N
Emission max.
Excitation max.
Peptide Sequence	DABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Irritant
Storage	-20°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
208771-1MGCN	04055977202854

Documentation

Calpain Substrate III, Fluorogenic - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

Calpain Substrate III, Fluorogenic - Calbiochem 分析证书

标题	批号
208771	输入批号

参考

参考信息概述
Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	28-May-2008 RFH
Synonyms	(DABCYL)-TPLK~SPPPSPR-(EDANS)
Description	A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with higher efficiency (k_cat/K_mx10^-2 (M^-1s^-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively). It is kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in intact Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
Form	Red lyophilized solid
Formulation	Supplied as a trifluoroacetate salt.
Intert gas (Yes/No)	Packaged under inert gas
Chemical formula	C₈₀H₁₁₄N₂₀O₁₈S
Peptide Sequence	DABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
Purity	≥98% by HPLC
Solubility	DMSO (5 mg/ml) or H₂O (1 mg/ml)
Storage	Protect from light -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Irritant
References	Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775.

种类

Life Science Research > Inhibitors and Biochemicals

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