Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.
Catalogue Number
70794
Brand Family
Novagen®
References
Product Information
•
2 x 100 ml
BugBuster Protein Extraction Reagent
•
10,000 U
Benzonase Nuclease, purity > 90%
•
10 ml
GST•Bind Resin
•
pkg/4
Chromatography Columns
•
2 x 100 ml
10X GST Bind/Wash Buffer
•
40 ml
10X Glutathione Reconstitution Buffer
•
1 g
Glutathione, Reduced
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 36/38
Irritating to eyes and skin.
S Phrase
S: 26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Bryan T. Carroll, et al. (2006) Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans. Journal of Biological Chemistry281, 35989-35996.
Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology46, 1073-1082.
