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203900
Sigma-AldrichBufalin - CAS 465-21-4 - Calbiochem
A cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na+,K+-ATPase activity (IC₅₀ = 1.4 nM).
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Bufalin - CAS 465-21-4 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
A cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na+,K+-ATPase activity (IC50 = 1.4 nM). Induces apoptosis in various human cell lines such as leukemia HL-60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na+,K+-ATPase inhibition. Reduces the level of topoisomerase II in human leukemia cells.
Catalogue Number
203900
Brand Family
Calbiochem®
Synonyms
5β, 20(22)-Bufadienolide-3β, 14-diol
References
References
Kurosawa, M., et al. 2000. Am. J. Physiol.278, C500. Kawazoe, N., et al. 1999. Oncogene 18, 2413. McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559. Watabe, M., et al. 1998. Oncogene 16, 779. Hashimoto, S., et al. 1997. Leuk. Res. 21, 875. Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599. Brownlee, A.A., et al. 1990. Clin. Sci.78, 169.
1.4 nM against ouabain-sensitive Na+,K+-ATPase activity
Purity
≥98% by HPLC
Physicochemical Information
Cell permeable
N
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
EI2962500
Safety Information
R Phrase
R: 26/27/28
Very toxic by inhalation, in contact with skin and if swallowed.
S Phrase
S: 22-36/37/39-45
Do not breathe dust. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Highly Toxic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
-20°C
Protect from Light
Protect from light
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Kurosawa, M., et al. 2000. Am. J. Physiol.278, C500. Kawazoe, N., et al. 1999. Oncogene 18, 2413. McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559. Watabe, M., et al. 1998. Oncogene 16, 779. Hashimoto, S., et al. 1997. Leuk. Res. 21, 875. Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599. Brownlee, A.A., et al. 1990. Clin. Sci.78, 169.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
22-May-2008 RFH
Synonyms
5β, 20(22)-Bufadienolide-3β, 14-diol
Description
A cardiotonic steroid isolated from Bufo species toads that potently inhibits ouabain-sensitive Na+,K+-ATPase activity. Induces apoptosis in various human cell lines such as leukemia HL60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na+-K+-ATPase inhibitors. Reduces the level of topoisomerase II in human leukemia cells.
Form
White to off-white solid
Intert gas (Yes/No)
Packaged under inert gas
CAS number
465-21-4
RTECS
EI2962500
Chemical formula
C₂₄H₃₄O₄
Structure formula
Purity
≥98% by HPLC
Solubility
DMSO (25 mg/ml) or warm Ethanol (25 mg/ml)
Storage
Protect from light
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Highly Toxic
References
Kurosawa, M., et al. 2000. Am. J. Physiol.278, C500. Kawazoe, N., et al. 1999. Oncogene 18, 2413. McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559. Watabe, M., et al. 1998. Oncogene 16, 779. Hashimoto, S., et al. 1997. Leuk. Res. 21, 875. Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599. Brownlee, A.A., et al. 1990. Clin. Sci.78, 169.
