Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Protect from Light
Protect from light
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
13-September-2007 RFH
Application
EIA (1:1,000) Tissue Staining (1:50 - 1:100)
Description
Avidin (and streptavidin) has 4 binding sites for biotin. Taking advantage of the high affinity between avidin and biotin, this fluorescein-conjugated biotin-X (a 6-atom spacer) provides a method for fluorescent detection of biotinylated antibodies.
Form
Lyophilized orange solid
Recommended reaction conditions
For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50-1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1000.
Solubility
DMF (5 mg/ml), DMSO (5 mg/ml), or Methanol (5 mg/ml)
Comments
Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Protect from light
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203
