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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

69041 Sigma-AldrichB834(DE3) Competent Cells - Novagen

B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

B834(DE3) Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
用户协议
Citations

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Description
Overview	B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with ³⁵S-methionine and selenomethionine for crystallography (2). DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Overview	Genotype: F^- ompT hsdS_B(r_B^- m_B^-) gal dcm met (DE3) This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number	69041
Brand Family	Novagen®

References
References	1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133. 2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.

Product Information
Components
Guaranteed efficiency	Guaranteed efficiency: > 2 x 10⁶ cfu/μg
Quality Level	MQ100

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
69041-3CN	04055977259711
69041-4CN	04055977259728

Documentation

B834(DE3) Competent Cells - Novagen MSDS

职位
物料安全数据表 (MSDS)

B834(DE3) Competent Cells - Novagen 分析证书

标题	批号
69041	输入批号

参考

参考信息概述
1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133. 2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.

小册子

标题
Competent Cell Brochure

引用

标题

Rossi, F., et al. 2008. Journal of Biological Chemistry 283, 3559.

Xue, X., et al. 2008. Journal of Virology 82, 2515.

Buetow, L., et al. 2007. Procedings of the National Academy of Science 104, 4321.

Alderwick, L.J., et al. 2006. Proceedings of the National Academy of Sciences (USA) 103, 2558.

Chédin, F., et al. 2006. Journal of Biological Chemistry 281, 18610.

Sasaki, H.M., et al. 2006. Procedings of the National Academy of Science 103, 14744.

Sheckler, L.R., et al. 2006. Journal of Biological Chemistry 281, 22896.

Song, F., et al. 2006. Journal of Biological Chemistry 281, 11028.

Alphey, M.S., et al. 2005. Journal of Biological Chemistry 280, 3068.

Dey, S., et al. 2005. Journal of Biological Chemistry 280, 14892.

Maria- Eugenia Guazzaroni, M-F., et al. 2005. Journal of Biological Chemistry 280, 20887.

Jogl, G., et al. 2005. i>Journal of Biological Chemistry 280, 738.

Li, H., et al. 2005. Protein Science 14, 3025.

Li, K., et al. 2005. Journal of Biological Chemistry 280, 29176.

Singh, S., et al. 2005. Journal of Biological Chemistry 280, 17101.

Zhu, G., et al. 2005. Journal of Biological Chemistry 280, 23057.

Chen, Y-h., et al. 2004. Nature. 429, 675.

Hsieh, T-J., et al. 2004. Journal of Biological Chemistry 279, 55587.

Lehmann, C., et al. 2004. Proteins: Structure, Function, and Bioinformatics 56, 376.

Mancini, E.J., et al. 2004. Acta Crystallographica D60, 588.

Nguyen, H., et al. 2004. Journal of Structural and Functional Genomics 5, 23.

James S. Parker, S. Mark Roe and David Barford. 2004. European Molecular Biology Organization Journal 23, 4727.

Welin, M., et al. 2004. Procedings of the National Academy of Science 101, 17970.

Obmolova, G., et al. 2000. Nature 407, 703.

David K. Worthylake, Kent L. Rossman and John Sondek. 2000. Nature 408, 682.

Conn, G.L., et al. 1999. Science 284, 1171.

Joey M. Studts and Brian G, Fox. 1999. Protein Expression and Purification 16, 109.

Cabral, J.H.M., et al. 1998. Cell 95, 649.

Fauman, E.B., et al. 1998. Cell 93, 617.

Gomis-Ruth, F.-X., et al. 1997. Nature 389, 77.

Dyda, F., et al. 1994. Science 266, 1981.

用户协议

标题
TB009 Competent Cells
TB053 Academic and Non-profit Laboratory Assurance Letter

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描述

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种类

Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression

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