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MABN798 Sigma-AldrichAnti-retSDR1 Antibody, clone A11

Anti-retSDR1 Antibody, clone A11 is an antibody against retSDR1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence.

Anti-retSDR1 Antibody, clone A11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Sensory & PNS Gene Symbol: DHRS3, RDH17, SDR16C1, UNQ2424/PRO4983 UniProt Number: O75911

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概述

Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, B	WB, IH(P), IF	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN798
Description	Anti-retSDR1 Antibody, clone A11
Alternate Names	Short-chain dehydrogenase/reductase 3 DD83.1 Retinol dehydrogenase 17 Retinal short-chain dehydrogenase/reductase 1 retSDR1 Short chain dehydrogenase/reductase family 16C member 1
Background Information	Short-chain dehydrogenase/reductase 3 (EC 1.1.1.300; UniProt O75911; also known as DD83.1, Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, retSDR1, Short chain dehydrogenase/reductase family 16C member 1) is encoded by the DHRS3 (also known as RDH17, SDR16C1) gene (ORF UNQ2424/PRO4983; Gene ID 9249) in human. The retSDR1 enzyme is an SDR (short-chain dehydrogenase/reductase) family member expressed in photoreceptor outer segments, where it mediates the reduction of all-trans-retinal for the regeneration of bleached pigments, the rate limiting step of retinoid reservoir replenishment during the visual cycle. Evidence indicates that retSDR1 also plays a role in tumor suppression. Alternation of vitamin A metabolism seems to be an important event in carcinogenesis and aberrant CpG-island methylation is reported in the DHRS3 promoter region in several neuroblastoma cell lines. The pro-apoptic p53 and p63 are both shown to regulate retSDR1 transcription.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-retSDR1 Antibody, clone A11 is an antibody against retSDR1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence.
Key Applications	Western Blotting Immunohistochemistry (Paraffin) Immunofluorescence
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected retSDR1 immunoreactivity in human and rat retina tissue sections. Immunofluorescence Analysis: Clone A11 hybridoma culture supernatant immunostained cone outer segments and a subset of inner nuclear layer somata in 4% paraformaldeyde-fixed, agarose-embedded bovine retina sections by fluorescent confocal microscopy (Courtesy of Francoise Haeseleer, Ph.D., University of Washington, Seattle, WA). Western Blotting Analysis: A representative lot detected a greatly upregulated retSDR1 protein expression following p53 or TAp63γ overexpression induction in DLD-1 human colorectal adenocarcinoma cells (Kirschner, R.D., et al. (2010). Cell Cycle. 9(11):2177-2188). Immunofluorescence Analysis: A representative lot immunostained cone, but not rod, outer segments in 4% paraformaldeyde-fixed, agarose-embedded bovine retina sections by fluorescent confocal microscopy. Immunoreactivity was also observed in a subset of somata localized in the inner nuclear layer (Haeseleer, F., et al. (1998). J. Biol. Chem. 273(34):21790-21799). Immunohistochemistry Analysis: A representative lot immunostained cone, but not rod, outer segments in 4% paraformaldeyde-fixed bovine retina whole mount sections. Immunoreactivity was also observed in a subset of somata localized in the inner nuclear layer (Haeseleer, F., et al. (1998). J. Biol. Chem. 273(34):21790-21799).

Biological Information
Immunogen	His-tagged recombinant full-length human retSDR1.
Epitope	Within a.a. 169-303.
Clone	A11
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone A11 recognized a truncated human retSDR1 comprising amino acid residues 169–303 by immunoblotting and immunostained cone, but not rod, outer segments by fluorescent confocal microscopy (Haeseleer, F., et al. (1998). J. Biol. Chem. 273(34):21790-21799). Epitope region is absent in human spliced isoform 2 (UniProt O75911-2).
Isotype	IgG1κ
Species Reactivity	Human Rat Bovine
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_004744
Gene Symbol	DHRS3 RDH17 SDR16C1 UNQ2424/PRO4983
Purification Method	Protein G Purified
UniProt Number	O75911
Molecular Weight	~33 kDa observed. 33.55/33.50/33.61 kDa (human/bovine/rat) calculated. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting of overexpressed human retSDR1 in HEK293 transfectant lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected the expression of full-length human retSDR1 in 10 µg of lysate from transfected, but not untransfected HEK293 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN798	04055977350364

Documentation

Anti-retSDR1 Antibody, clone A11 MSDS

职位
物料安全数据表 (MSDS)

Anti-retSDR1 Antibody, clone A11 分析证书

标题	批号
Anti-retSDR1, clone A11 -Q2680117	Q2680117

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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