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AB3849-I Sigma-AldrichAnti-phospho SMAD2 Antibody (Ser465/467)

Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.

Anti-phospho SMAD2 Antibody (Ser465/467) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: SMAD2, MADH2, MADR2 UniProt Number: Q15796

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB3849-I
Replaces	AB3849
Description	Anti-phospho SMAD2 Antibody (Ser465/467)
Alternate Names	Mothers against decapentaplegic homolog 2, Ser465/467 phosphorylated hMAD-2, Ser465/467 phosphorylated hSMAD2, Ser465/467 phosphorylated JV18-1, Ser465/467 phosphorylated MAD homolog 2, Ser465/467 phosphorylated Mad-related protein 2, Ser465/467 phosphorylated Mothers against DPP homolog 2, Ser465/467 phosphorylated SMAD 2, Ser465/467 phosphorylated SMAD family member 2, Ser465/467 phosphorylated Smad2, Ser465/467 phosphorylated
Background Information	Mothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events.

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.
Key Applications	Western Blotting

Biological Information
Immunogen	KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
Epitope	Phospho-Ser465/467.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	This antibody detects Smad2 Ser465/467 phosphorylation in TGFbeta-stimulated, but not unstimulated HepG2 cells. The corresponding target phosphoserine residues in the alternatively spliced human Smad2Deltaexon3 (Isoform Short) are pSer435 & pSer437 (UniProt Q15796-2).
Species Reactivity	Human
Species Reactivity Note	Human. Predicted to react with Mouse, Rat, Bovine, Xenopus, Zebrafish, Chicken based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_005892
Gene Symbol	SMAD2 MADH2 MADR2
Modifications	Phosphorylation Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	Q15796
Molecular Weight	~53 kDa. 52.31 kDa (Isoform Long) and 48.96 kDa (Isoform Short; Smad2Deltaexon3) calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 µg lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
AB3849-I	04055977333800

Documentation

Anti-phospho SMAD2 Antibody (Ser465/467) MSDS

职位
物料安全数据表 (MSDS)

Anti-phospho SMAD2 Antibody (Ser465/467) 分析证书

标题	批号
Anti-phospho SMAD2 (Ser465/467) - 3172383	3172383
Anti-phospho SMAD2 (Ser465/467) - 3233096	3233096
Anti-phospho SMAD2 (Ser465/467) - 3384842	3384842
Anti-phospho SMAD2 (Ser465/467) - 3451051	3451051
Anti-phospho SMAD2 (Ser465/467) - 3532294	3532294
Anti-phospho SMAD2 (Ser465/467) - 3753293	3753293
Anti-phospho SMAD2 (Ser465/467) - 3910141	3910141
Anti-phospho SMAD2 (Ser465/467) - 3993464	3993464
Anti-phospho SMAD2 (Ser465/467) - 4140613	4140613
Anti-phospho SMAD2 (Ser465/467) -2730005	2730005

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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