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MABE350 Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

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MABE350
100 µg  
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      概述

      Replacement Information

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ChIPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE350
      DescriptionAnti-phospho-RNAPII (Tyr1) Antibody, clone 3D12
      Alternate Names
      • DNA-directed RNA polymerase II subunit RPB1
      • RNA polymerase II subunit B1
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA-directed RNA polymerase II subunit RPB1
      Background InformationRNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
      References
      Product Information
      FormatPurified
      Control
      • Untreated and lambda phosphatase treated HeLa cell lysates
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1.
      Clone3D12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POLR2A
      • POLR2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~220 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot Analysis in untreated and lambda phosphatase treated HeLa cell lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MABE350 04053252833007

      Documentation

      Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12 MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12 分析证书

      标题批号
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - Q2115839 Q2115839
      Anti-phospho-RNAPII (Tyr1), -2812309 2812309
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 2705155
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 3173540 3173540
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 3223358 3223358
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 3388664 3388664
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 3462117 3462117
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 3506606 3506606
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 3587268 3587268
      Anti-phospho-RNAPII (Tyr1), clone 3D12 - 3766809 3766809

      参考

      参考概述公共医疗ID
      CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II.
      Mayer, Andreas, et al.
      Science, 336: 1723-5 (2012)  2012

      显示摘要
      22745433 22745433

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      种类

      Life Science Research > Antibodies and Assays > Primary Antibodies