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MABE954 Anti-phospho RNA Pol II (Ser5), clone 1H4B6 Antibody

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MABE954
100 µg  
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      概述

      Replacement Information

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ca, Mk, M, RWB, ChIP-seq, ICC, ELISA, ChIPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE954
      DescriptionAnti-phospho RNA Pol II (Ser5), clone 1H4B6 Antibody
      Alternate Names
      • DNA-directed RNA polymerase II subunit RPB1
      • RNA polymerase II subunit B1
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA-directed RNA polymerase II subunit RPB1
      Background InformationRNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription. As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho RNA Pol II (Ser5) antibody, clone 1H4B6 is validated for use in western blotting, ChIP-seq, ICC, ELISA & ChIP for the detection of phospho RNA Pol II (Ser5).
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Immunocytochemistry
      • Enzyme Immunoassay (ELISA)
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected phospho RNA Pol II (Ser5) in 10 µg of HeLa nuclear extract.

      Western Blotting: A representative lot detected phospho RNA Pol II (Ser5) in 10 µg of HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.).

      Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser5) in HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.; Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

      Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser5) in HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.; Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

      ELISA Analysis: A representative lot from an independent laboratory detected phospho RNA Pol II (Ser5) in a panel of unmodified and modified RNA Pol II (Ser5) proteins (Prof. Taro Tachibana, Cell Engineering Corporation).

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot from an independent laboratory detected phospho RNA Pol II (Ser5) in HeLa cells (Prof. Taro Tachibana, Cell Engineering Corporation).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human RNA PoI II phosphorylated at Ser5.
      Clone1H4B6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Canine
      • Monkey
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POLR2A
      • POLR2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~217 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho RNA Pol II (Ser5) in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MABE954 04053252970627

      Documentation

      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 Antibody MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 Antibody 分析证书

      标题批号
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - Q2399138 Q2399138
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3206358 3206358
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3241218 3241218
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3257044 3257044
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3284323 3284323
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3326205 3326205
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3379353 3379353
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3426771 3426771
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3464589 3464589
      Anti-phospho RNA Pol II (Ser5), clone 1H4B6 - 3475671 3475671

      参考

      参考概述公共医疗ID
      A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples.
      Maehara, Kazumitsu, et al.
      Nucleic Acids Res., 41: 54-62 (2013)  2013

      显示摘要
      23125363 23125363
      The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach.
      Odawara, Jun, et al.
      BMC Genomics, 12: 516 (2011)  2011

      显示摘要
      22011111 22011111

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      种类

      Life Science Research > Antibodies and Assays > Primary Antibodies