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This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
More>>This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
References
Product Information
Format
AlexaFluor®647
HS Code
3002 15 90
Presentation
Purified mouse conjugate in buffer containing PBS, 15 mg/ml BSA and 0.1% sodium azide.
This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
Key Applications
Immunocytochemistry
Biological Information
Immunogen
Linear peptide corresponding to phospho Histone H2A.X (Ser139).
Clone
JBW301
Concentration
0.5 mg/mL
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with vertebrates based on 100% sequence homology.
~17 kDa observed. Refer to Cat. No. 05-636 for observed molecular weight information.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in untreated and staurosporin treated HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.
Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
EMD Millipore's Alexa Fluor® Conjugated Histone and puromycin antibodies are validated in immunocytochemistry. All EMD Millipore antibodies are based on the expertise of Upstate & Chemicon. 详细了解 >>
