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This AlexaFluor®647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.
More>>This AlexaFluor®647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.
References
Product Information
Format
AlexaFluor®647
Presentation
Purified conjugated rabbit polyclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
This AlexaFluor®647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.
Key Applications
Immunocytochemistry
Application Notes
See data sheet for cat# 07-164, Anti-phospho H2A.X (Ser139) for additional Western blot, Immnoprecipitation, and ChIP-seq analysis performed using an unconjugated version of this antibody.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to phospho H2A.X (Ser139).
Epitope
Phosphorylated Ser139
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Recognizes Histone H2A.X phosphorylated at Serine 139.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Vertebrates based on 100% sequence homology.
A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in HeLa cells. Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
