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ABS1008 Sigma-AldrichAnti-phospho Glucocorticoid Receptor (Ser134) Antibody

Anti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书

Research Category: Signaling Research Sub-Category: Hormones & Receptors Gene Symbol: NR3C1, GCCR, GCR, GCRST, GRL UniProt Number: P04150

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概述

Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABS1008
Description	Anti-phospho Glucocorticoid Receptor (Ser134) Antibody
Alternate Names	Glucocorticoid receptor, Ser134-phosphorylated GR, Ser134-phosphorylated Nuclear receptor subfamily 3 group C member 1, Ser134-phosphorylated
Background Information	Glucocorticoid receptor (UniProt P04150; also known as GR, Nuclear receptor subfamily 3 group C member 1) is encoded by the NR3C1 (also known as GCCR, GCR, GCRST, GRL) gene (Gene ID 2908) in human. Glucocorticoid receptor is widely expressed in almost all cell types and regulates genes that control development, metabolism, and immune response upon binding glucocorticoids. Hormone-bound GR translocates from the cytoplasm to the nucleus where it regulates gene transcriptions by binding to glucocorticoid response elements (GREs) or by interacting with various transcription factors. Alternatively, hormone-bound GR can represses gene transcriptions by blocking the nuclear translocation of transcription factors. BDNF-stimulated TrkB activation is shown to induce GR phosphorylation at Ser155 and Ser287 in primary rat cortical neurons (equivalent to human GR Ser134 and Ser267), resulting in enhanced GR transcription activity. GR is composed of an N-terminal modulating domain (a.a. 1-420), a DNA-binding domain (a.a. 421-486), and a C-terminal ligand-binding domain (a.a. 528-777).

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal antibody in Tris-buffered saline (pH 8.0) with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.
Key Applications	Western Blotting
Application Notes	Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of endogenous GR in primary rat cortical neurons (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714). Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of exogenously expressed wild-type, but not S155A mutant, rat GR in transfected HEK293TrkB cells (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).

Biological Information
Immunogen	Linear peptide corresponding to a human glucocorticoid receptor sequence containing phosphorylated Ser134.
Epitope	pSer134.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	The target phosphorylation site recognized by this polyclonal antibody is present in all 9 human GR spliced isoforms (Ser108 of isoforms Alpha-B and Beta-B; Ser134 of all the other isoforms) and in both rat GR spliced isoforms (Ser155 of isoform A and Ser128 of isoform B) reported by UniProt (P04150 and P06536, respectively).
Species Reactivity	Human Rat
Species Reactivity Note	Human, Rat. Predicted to react with Canine, Horse, Feline, Sheep based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_000167
Gene Symbol	NR3C1 GCCR GCR GCRST GRL
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	P04150
Molecular Weight	~85 kDa observed. 87.56 kDa (Rat isoforms A), 84.83 kDa (Rat isoforms B), 85.66 kDa (Human isoform Alpha; Alpha-A), 81.51 kDa (Human isoform Beta; Beta-A), 85.82 kDa (Human isoform Alpha-2; Gamma), 81.67 kDa (Human isoform Beta-2), 64.75 kDa (Human isoform GR-A alpha), 60.60 kDa (Human isoform GR-A beta), 82.85 kDa (Human isoform Alpha-B), 78.70 kDa (Human isoform Beta-B), 82.90 kDa (Human isoform 10; hGRDelta313-338) calculated. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in cell lysate from HEK293 expressing FLAG-tagged human glucocorticoid receptor. Western Blotting Analysis: 0.2 µg/mL of this antibody detected Ser134 phosphorylation of FLAG-tagged human glucocorticoid receptor in 10 ug lysate from BDNF-treated HEK293 transfectants.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABS1008	04055977334234

Documentation

Anti-phospho Glucocorticoid Receptor (Ser134) Antibody 分析证书

标题	批号
Anti-phospho Glucocorticoid Receptor (Ser134) - 3174766	3174766
Anti-phospho Glucocorticoid Receptor (Ser134) - 3520924	3520924
Anti-phospho Glucocorticoid Receptor (Ser134) - 4203375	4203375
Anti-phospho Glucocorticoid Receptor (Ser134) -Q2668259	Q2668259

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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