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MABS155 Sigma-AldrichAnti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1

Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: Hormones & Receptors Gene Symbol: ADRBK1, BARK, BARK1, GRK2 UniProt Number: P25098

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP, PIA	M	Unconjugated	Monoclonal Antibody

Description
Catalogue Number	MABS155
Description	Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1
Alternate Names	Beta-adrenergic receptor kinase 1 Beta-ARK-1 G-protein coupled receptor kinase 2
Background Information	G-protein coupled receptor kinase 2 (Grk2), also known as BARK or BARK1, is a member of the large family of GRK kinases that phosphorylate G-protein coupled receptors (GPCR), resulting in the recruitment of arrestins to the cytoplasmic domains of GPCR’s and subsequent internalization of these receptors by endocytosis. GRK2 is ubiquitously expressed, and modulates diverse cellular processes through association with a number of non-receptor substrates. GRK2 regulates actin dynamics and cell motililty through interaction with radixin, tubilin, and ezrin. In addition, Grk2 may play a role in the signaling of TGFβ by blocking the nuclear translocation of Smad2 and Smad3 proteins. It has also been shown that Grk2 may play a role in the hedgehog signaling pathway during embryonic development, by modulating the signaling of the atypical G-protein coupled receptor Smo. Grk2 is inactivated by phosphorylation of ser670 by ERK. It is also phosphorylated by PKC and Src, depending on the cellular context.

References

Product Information
Format	Unconjugated
Control	Untreated and Calyculin A/Okadaic Acid treated A431 cells
Presentation	Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.
Key Applications	Western Blotting Immunoprecipitation Peptide Inhibition Assay
Application Notes	Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot blocked phospho-GRK2 in 10 µg of Calyculin A/Okadaic Acid treated and untreated Jurkat cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-GRK2 in HeLa cell lysate.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human GRK2 phosphorylated at Ser670.
Epitope	Phosphorylated Ser670
Clone	15G11.1
Host	Mouse
Specificity	This antibody recognizes GRK2 phosphorylated at Ser670.
Isotype	IgG2bκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001610
Gene Symbol	ADRBK1 BARK BARK1 GRK2
Purification Method	Protein G Purified
UniProt Number	P25098
Molecular Weight	~70 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in untreated and Calyculin A/Okadaic Acid treated A431 cells. Western Blot Analysis: A 1:1,000 dilution of this antibody detected GRK2 in 10 µg of untreated and Calyculin A/Okadaic Acid treated A431 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABS155	04053252440854

Documentation

Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 MSDS

职位
物料安全数据表 (MSDS)

Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 分析证书

标题	批号
Anti-phospho-GRK2 (Ser670), -2576404	2576404
Anti-phospho-GRK2 (Ser670), -2640559	2640559
Anti-phospho-GRK2 (Ser670), -2686168	2686168
Anti-phospho-GRK2 (Ser670), -2718264	2718264
Anti-phospho-GRK2 (Ser670), -2792488	2792488
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 2298450	2298450
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 3423237	3423237
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 4162702	4162702
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - Q2032354	Q2032354
Anti-phospho-GRK2 (Ser670), clone 15G11.1 Monoclonal Antibody	3050683

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种类

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