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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABC118 Sigma-AldrichAnti-phospho Beclin Antibody (Thr119)

Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.

Anti-phospho Beclin Antibody (Thr119) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: BECN1 UniProt Number: Q14457

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, PIA	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABC118
Description	Anti-phospho Beclin Antibody (Thr119)
Alternate Names	Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197
Background Information	The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.

References

Product Information
Format	Affinity Purified
Control	Human prostate tumor tissue lysate.
Presentation	Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.
Key Applications	Western Blotting Peptide Inhibition Assay
Application Notes	Western Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.
Epitope	BH3 domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Chicken, Porcine, Xenopus based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_003757
Gene Symbol	BECN1
Purification Method	Affinity Purfied
UniProt Number	Q14457
Molecular Weight	~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in human prostate tumor tissue lysate. Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABC118	04053252909290

Documentation

Anti-phospho Beclin Antibody (Thr119) MSDS

职位
物料安全数据表 (MSDS)

Anti-phospho Beclin Antibody (Thr119) 分析证书

标题	批号
Anti-phospho Beclin (Thr119) - 2836178	2836178
Anti-phospho Beclin (Thr119) - 3180445	3180445
Anti-phospho Beclin (Thr119) - 3454399	3454399
Anti-phospho Beclin (Thr119) - 3524337	3524337
Anti-phospho Beclin (Thr119) - 3855747	3855747
Anti-phospho Beclin (Thr119) - 4078587	4078587
Anti-phospho Beclin (Thr119) - 4215024	4215024
Anti-phospho Beclin (Thr119) - Q2196317	Q2196317
Anti-phospho Beclin (Thr119) -2527587	2527587
Anti-phospho Beclin (Thr119) -2742257	2742257

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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