数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP43 Sigma-AldrichAnti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
小册子
Data Sheet
Citations

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Fe, H	M	Monoclonal Antibody

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
OP43-100UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	100 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹
OP43-20UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	20 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
Catalogue Number	OP43
Brand Family	Calbiochem®
Application Data	Detection of human p53 by immunoblotting. Sample: Whole cell lysates from A431 cells. Primary antibody: Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) (Cat. No. OP43) (1 μg/ml). Detection: chemiluminescence.

References
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Legros, Y., et al. 1994. Oncogene 9, 2071. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

References

El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative control	SK-OV-3 cells or normal skin tissue
Positive control	A431 cells or breast carcinoma tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoprecipitation and Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation Paraffin Sections
Application Notes	Frozen sections (1 µg/ml; see application references) Immunoblotting (0.1-1 µg/ml; see application references) Immunocytochemistry (1-2.5 µg/ml; see application references) Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references) Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
Application Comments	Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with ³⁵S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	wild type, recombinant, human p53
Immunogen	Human
Epitope	Within amino acids 21-25 of human p53
Clone	DO-1
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Feline Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP43-100UGCN	04055977227543
OP43-20UGCN	04055977227123

Documentation

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) 分析证书

标题	批号
OP43	输入批号

参考

参考信息概述
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Legros, Y., et al. 1994. Oncogene 9, 2071. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

参考信息概述

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

引用

标题

Fang Zhang, et al. (2006) Ribosomal stress couples the unfolded protein response to p53-dependent cell cycle arrest. Journal of Biological Chemistry 281, 30036-30045.

Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264.

Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503.

Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	05-June-2008 JSW
Application	Frozen sections (1 µg/ml; see application references) Immunoblotting (0.1-1 µg/ml; see application references) Immunocytochemistry (1-2.5 µg/ml; see application references) Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references) Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
Application Data	Detection of human p53 by immunoblotting. Sample: Whole cell lysates from A431 cells. Primary antibody: Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) (Cat. No. OP43) (1 μg/ml). Detection: chemiluminescence.
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
Background	The human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death.
Host	Mouse
Immunogen species	Human
Immunogen	wild type, recombinant, human p53
Epitope	Within amino acids 21-25 of human p53
Clone	DO-1
Isotype	IgG_2a
Species	feline, human, not mouse, not rat
Positive control	A431 cells or breast carcinoma tissue
Negative control	SK-OV-3 cells or normal skin tissue
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with ³⁵S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Legros, Y., et al. 1994. Oncogene 9, 2071. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
Citation	Fang Zhang, et al. (2006) Ribosomal stress couples the unfolded protein response to p53-dependent cell cycle arrest. Journal of Biological Chemistry 281, 30036-30045. Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264. Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503. Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.
Application references	Immunoprecipitation and Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.

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描述

OP43L

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

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Life Science Research > Antibodies and Assays > Primary Antibodies

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