数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP33 Sigma-AldrichAnti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
小册子
Data Sheet
Citations

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, H, M, Pm, R	M	Monoclonal Antibody

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
OP33-100UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	100 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹
OP33-20UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	20 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
Catalogue Number	OP33
Brand Family	Calbiochem®

References
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304. Ball, R.K., et al. 1984. EMBO J. 3, 1485.

References

El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Ball, R.K., et al. 1984. EMBO J. 3, 1485.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive control	Hs27 cells or breast carcinoma tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
Key Applications	Frozen Sections Immunofluorescence Immunoprecipitation Not Western Blot Paraffin Sections
Application Notes	Frozen Sections (see applications references) Immunoblotting (not recommended) Immunofluorescence (1-20 μg/ml) Immunoprecipitation (1 μg per sample) Paraffin Sections (5 μg/ml, heat pre-treatment required)
Application Comments	Wild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with ³⁵S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	vLM mouse tumor cells
Immunogen	Mouse
Clone	PAb1620
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Bovine Human Mouse Primate Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP33-100UGCN	04055977208443
OP33-20UGCN	07790788053901

Documentation

Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) 分析证书

标题	批号
OP33	输入批号

参考

参考信息概述
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304. Ball, R.K., et al. 1984. EMBO J. 3, 1485.

参考信息概述

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

引用

标题

Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Application	Frozen Sections (see applications references) Immunoblotting (not recommended) Immunofluorescence (1-20 μg/ml) Immunoprecipitation (1 μg per sample) Paraffin Sections (5 μg/ml, heat pre-treatment required)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~53 kDa wild-type p53 protein.
Background	The human p53 tumor suppressor gene encodes a 393 amino acid phospho-protein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
Host	Mouse
Immunogen species	Mouse
Immunogen	vLM mouse tumor cells
Clone	PAb1620
Isotype	IgG_2a
Species	bovine, human, mouse, primate, rat
Positive control	Hs27 cells or breast carcinoma tissue
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Wild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with ³⁵S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304. Ball, R.K., et al. 1984. EMBO J. 3, 1485.
Citation	Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.
Application references	Immunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.

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种类

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