数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP104L Sigma-AldrichAnti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

This Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) is validated for use in Immunoblotting, IP, Frozen Sections, IF Paraffin Sections for the detection of p53 (Ab-11) (Pantropic).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
Catalogue Number	OP104L
Brand Family	Calbiochem®

References
References	Levine, A.J. 1997. Cell 88, 323. Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194. Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209. Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992. Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493. Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129. Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455. Waldman, T., et al. 1995. Cancer Res. 55, 5187. Clarke, A.R., et al. 1994. Oncogene 9, 1767. Cho, Y., et al. 1994. Science 265, 346. El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Lane, D.P. 1992. Nature 358, 15.

References

Levine, A.J. 1997. Cell 88, 323.
Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Clarke, A.R., et al. 1994. Oncogene 9, 1767.
Cho, Y., et al. 1994. Science 265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Lane, D.P. 1992. Nature 358, 15.

Product Information
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 100 µg BSA.
Negative control	SK-OV-3 cells
Positive control	A431 or SK-BR-3 cells
Preservative	None
Quality Level	MQ100

Applications
Application References	Immunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
Key Applications	Immunoblotting (Western Blotting) Immunoprecipitation Not Frozen Sections Not Immunofluorescence Not Paraffin Sections
Application Notes	Immunoblotting (2.5 µg/ml, chemiluminescence, see application references) Immunoprecipitation (1 µg/reaction, see comments, see application references) Frozen Sections (not recommended) Immunofluorescence (not recommended) Paraffin Sections (not recommended)
Application Comments	This antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant, human p53 protein
Immunogen	Human
Epitope	within amino acids 306-393 of human p53
Clone	PAb1802
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP104L-100UGCN	04055977224917

Documentation

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) MSDS

职位
物料安全数据表 (MSDS)

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) 分析证书

标题	批号
OP104L	输入批号

参考

参考信息概述
Levine, A.J. 1997. Cell 88, 323. Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194. Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209. Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992. Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493. Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129. Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455. Waldman, T., et al. 1995. Cancer Res. 55, 5187. Clarke, A.R., et al. 1994. Oncogene 9, 1767. Cho, Y., et al. 1994. Science 265, 346. El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Lane, D.P. 1992. Nature 358, 15.

参考信息概述

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-October-2007 RFH
Application	Immunoblotting (2.5 µg/ml, chemiluminescence, see application references) Immunoprecipitation (1 µg/reaction, see comments, see application references) Frozen Sections (not recommended) Immunofluorescence (not recommended) Paraffin Sections (not recommended)
Description	Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Background	p53 (393 amino acids) exhibits sequence-specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage by inducing GADD45, WAF1, and MDM2. p53 is the most commonly mutated gene in human cancer with most mutations occurring in the DNA contact residues. p53 binds damaged DNA non-specifically via its C-terminal 75 amino acids which in turn may be responsible for subsequent activation, sequence-specific DNA binding, and WAF1 induction allowing DNA repair to take place. p53 down regulates bcl-2 expression and up regulates bax expression, but may not always be necessary for apoptosis. In addition to the afore mentioned proteins, two other proteins involved in replication and recombination, RPA and RAD51, also bind p53.
Host	Mouse
Immunogen species	Human
Immunogen	recombinant, human p53 protein
Epitope	within amino acids 306-393 of human p53
Clone	PAb1802
Isotype	IgG₁
Species	human, mouse
Positive control	A431 or SK-BR-3 cells
Negative control	SK-OV-3 cells
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 100 µg BSA.
Preservative	None
Comments	This antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
Toxicity	Standard Handling
References	Levine, A.J. 1997. Cell 88, 323. Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194. Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209. Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992. Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493. Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129. Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455. Waldman, T., et al. 1995. Cancer Res. 55, 5187. Clarke, A.R., et al. 1994. Oncogene 9, 1767. Cho, Y., et al. 1994. Science 265, 346. El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Lane, D.P. 1992. Nature 358, 15.
Application references	Immunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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