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MABN1833 Sigma-AldrichAnti-nAChR α4 Subunit Antibody, clone 369

Anti-nAChR α4 Subunit Antibody, clone 369 is an antibody against nAChR α4 Subunit for use in Immunohistochemistry (Paraffin), Immunofluorescence.

Anti-nAChR α4 Subunit Antibody, clone 369 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: CHRNA4, NACHRA4, NACRA4 UniProt Number: P43681

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Rb, Mk	IH(P), IF	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN1833
Description	Anti-nAChR α4 Subunit Antibody, clone 369
Alternate Names	Neuronal acetylcholine receptor subunit alpha-4 alpha4 nAChR subunit Cholinergic receptor, nicotinic, alpha polypeptide 4 nAChR alpha4 subunit Neuronal nicotinic acetylcholine receptor alpha-4 subunit
Background Information	Neuronal acetylcholine receptor subunit alpha-4 (UniProt P43681; also known as alpha4 nAChR subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit) is encoded by the CHRNA4 (also known as NACRA4, NACHRA4) gene (Gene ID 1137) in human. The nicotinic acetylcholine (ACh) receptors (nAChRs) are activated by acetylcholine (ACh) and nicotinic agonists, such as cytisine, 1,1-dimethyl-4-phenylpiperazinium and carbachol. The nAChRs are ligand-gated pentameric cation channels. Currently, 12 neuronal and 5 muscle nAChR subunits have been identified. The nAChRs can exist as homopentamers of five identical alpha subunits (α7, α8, or α9), alpha heteropentamers composed of more than one type of alpha subunits (α7-α10), or as alpha/beta heteropentamers consisting of various combinations of alpha (α2-α6) and beta (β2-β4) subunits. The heteromeric alpha/beta types display high ligand affinity, but are insensitive to α-bungarotoxin (αBgt), while the alpha-only homomeric and heteromeric pentamers show lower agonist affinity, but high αBgt sensitivity. The α4β2 (two α4 and three β2 subunits) and α7 (five α7 subunits) nAChRs are the predominant nAChRs in the central nervous system (CNS) among mammalian species. The alpha-4 subunit is initially produced with a signal peptide (a.a. 1-28), the removal of which yields the mature 627-amino acid membrane protein with a large extracellular domain (a.a. 29-242), four transmembrane segments (a.a. 243-267, 275-293, 309-330, 601-619), and a large cytoplasmic loop (a.a. 331-600) between the third and fourth transmembrane domains.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-nAChR α4 Subunit Antibody, clone 369 is an antibody against nAChR α4 Subunit for use in Immunohistochemistry (Paraffin), Immunofluorescence.
Key Applications	Immunohistochemistry (Paraffin) Immunofluorescence
Application Notes	Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected nAChR α4 Subunit in human small intestine tissue. Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in monkey frozen retina sections by fluorescence immunohistochemistry (Liu, J., et al. (2009). Invest. Ophthalmol. Vis .Sci. 50(3):1408-1415). Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in various areas of the rabbit brainstem by fluorescence immunohistochemical staining of free-floating sections (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12). Immunofluorescence Analysis: A represenative lot detected an increase of nAChR α4-positive neurons in the A1, A2 and A6 brainstem noradrenergic areas of female rabbits 1 hr after mating by fluorescence immunohistochemical staining of free-floating sections. The number of nAChR α4-positive neurons subsided to basal level 2 hrs after mating (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).

Biological Information
Immunogen	Recombinant human nAChR α4 subunit.
Clone	369
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human Rabbit Monkey
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000735
Gene Symbol	CHRNA4 NACHRA4 NACRA4
Purification Method	Protein G Purified
UniProt Number	P43681
Molecular Weight	67.16 kDa calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry in human cerebellum tissue. Immunohistochemistry Analysis: An 1:50 dilution of this antibody detected nAChR α4 Subunit in human cerebellum tissue.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN1833	04055977329537

Documentation

Anti-nAChR α4 Subunit Antibody, clone 369 MSDS

职位
物料安全数据表 (MSDS)

Anti-nAChR α4 Subunit Antibody, clone 369 分析证书

标题	批号
Anti-nAChR α4 Subunit, clone 369 - 4178989	4178989
Anti-nAChR α4 Subunit, clone 369 -Q2650574	Q2650574

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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