数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP23 Sigma-AldrichAnti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1)

This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
OP23-100UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	100 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹
OP23-200UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	200 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
Catalogue Number	OP23
Brand Family	Calbiochem®

References
References	Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s. Sidransky, D., et al. 1992. Science 256, 102. Bos, J.L. 1989. Cancer Res. 49, 4682. Furth, M. E., et al. 1987. Oncogene 1, 47. Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384. Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

References

Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Positive control	Normal skin tissue or ras 1 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (5 µg/ml) Immunoblotting (10 µg/ml) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (5 µg/ml) Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Application Comments	For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant p21 Ras
Epitope	within amino acids 54-188
Clone	F235-1.7.1
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP23-100UGCN	04055977208962
OP23-200UGCN	04055977208986

Documentation

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) MSDS

职位
物料安全数据表 (MSDS)

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) 分析证书

标题	批号
OP23	输入批号

参考

参考信息概述
Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s. Sidransky, D., et al. 1992. Science 256, 102. Bos, J.L. 1989. Cancer Res. 49, 4682. Furth, M. E., et al. 1987. Oncogene 1, 47. Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384. Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Application	Frozen Sections (5 µg/ml) Immunoblotting (10 µg/ml) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (5 µg/ml) Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.
Background	The human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-Ras are the homologues of v-H- and v-K-Ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and their relevance to human disease diagnosis and prognosis is still emerging.
Host	Mouse
Immunogen	recombinant p21 Ras
Epitope	within amino acids 54-188
Clone	F235-1.7.1
Isotype	IgG₁
Species	human, mouse, rat
Positive control	Normal skin tissue or ras 1 cells
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s. Sidransky, D., et al. 1992. Science 256, 102. Bos, J.L. 1989. Cancer Res. 49, 4682. Furth, M. E., et al. 1987. Oncogene 1, 47. Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384. Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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