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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP17L Sigma-AldrichAnti-c-Fos (Ab-1) Mouse mAb (2G9C3)

This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.
Catalogue Number	OP17L
Brand Family	Calbiochem®

References
References	Jensen, D.E., et al. 1991. Oncogene 6, 1219. Reiss, M., et al. 1990. Cancer Res. 50, 6641. RouscherIII, F.J., et al. 1988. Science 240, 1010. Sassone-Corsi, P., et al. 1988. Cell 54, 553. Giardina, S.L., et al. 1987. Anal Biochem. 161, 109. Verma, I.M., et al. 1987. Cancer Res. 49, 29. Verma, I.M., et al. 1987. Cell 51, 513. Muller, R. 1986. Viochem. Biophys. Acta. 823, 207. Verma, P. 1986. Trends Genet. 2, 93. Greenberg, M., et al. 1984. Nature (London) 311, 433.

References

Jensen, D.E., et al. 1991. Oncogene 6, 1219.
Reiss, M., et al. 1990. Cancer Res. 50, 6641.
RouscherIII, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res. 49, 29.
Verma, I.M., et al. 1987. Cell 51, 513.
Muller, R. 1986. Viochem. Biophys. Acta. 823, 207.
Verma, P. 1986. Trends Genet. 2, 93.
Greenberg, M., et al. 1984. Nature (London) 311, 433.

Product Information
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 200 µg BSA.
Positive control	HeLa cells
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Not Paraffin Sections
Application Notes	Immunoblotting (2.5-5 µg/ml) Immunocytochemistry (2.5-5 µg/ml) Paraffin Sections (not recommended)
Application Comments	Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
Immunogen	Human
Clone	2G9C3
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP17L-200UGCN	04055977208818

Documentation

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) MSDS

职位
物料安全数据表 (MSDS)

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) 分析证书

标题	批号
OP17L	输入批号

参考

参考信息概述
Jensen, D.E., et al. 1991. Oncogene 6, 1219. Reiss, M., et al. 1990. Cancer Res. 50, 6641. RouscherIII, F.J., et al. 1988. Science 240, 1010. Sassone-Corsi, P., et al. 1988. Cell 54, 553. Giardina, S.L., et al. 1987. Anal Biochem. 161, 109. Verma, I.M., et al. 1987. Cancer Res. 49, 29. Verma, I.M., et al. 1987. Cell 51, 513. Muller, R. 1986. Viochem. Biophys. Acta. 823, 207. Verma, P. 1986. Trends Genet. 2, 93. Greenberg, M., et al. 1984. Nature (London) 311, 433.

参考信息概述

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Application	Immunoblotting (2.5-5 µg/ml) Immunocytochemistry (2.5-5 µg/ml) Paraffin Sections (not recommended)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.
Background	The proto-oncogene fos has been implicated in cell growth, differentiation, and development. Proto-oncogene fos is induced by a large number of stimuli ranging from mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
Host	Mouse
Immunogen species	Human
Immunogen	a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
Clone	2G9C3
Isotype	IgG_2a
Species	human, mouse, rat
Positive control	HeLa cells
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 200 µg BSA.
Preservative	None
Comments	Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.
Toxicity	Standard Handling
References	Jensen, D.E., et al. 1991. Oncogene 6, 1219. Reiss, M., et al. 1990. Cancer Res. 50, 6641. RouscherIII, F.J., et al. 1988. Science 240, 1010. Sassone-Corsi, P., et al. 1988. Cell 54, 553. Giardina, S.L., et al. 1987. Anal Biochem. 161, 109. Verma, I.M., et al. 1987. Cancer Res. 49, 29. Verma, I.M., et al. 1987. Cell 51, 513. Muller, R. 1986. Viochem. Biophys. Acta. 823, 207. Verma, P. 1986. Trends Genet. 2, 93. Greenberg, M., et al. 1984. Nature (London) 311, 433.

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产品目录编号

描述

OP17

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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