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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP16 Sigma-AldrichAnti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)

Anti-c-ErbB2/c-Neu (Ab-4), mouse monoclonal, clone 7.16.4, recognizes ~185 kDa c-ErbB2 in overexpressing B104-1-1 cells. It is validated for WB, IP, ICC, frozen sections, & growth inhibition assay.

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

同义词: Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.
Catalogue Number	OP16
Brand Family	Calbiochem®
Synonyms	Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
Application Data	Detection of rat c-neu by immunocytochemistry. Sample: B104-1-1 cells overexpressing c-neu. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) (Cat. No. OP16) (1.4 µg/ml). Detection: fluorescence.

References
References	Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15. Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239. DiFiore, P.P., et al. 1987. Science 237, 178. Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Slamon, D.J., et al. Science 235, 177. Varley, J.M., et al. 1987. Oncogene 1, 423. Bargmann, C.I., et al. 1986. Nature 319, 226. Yamamoto, T., et al. 1986. Nature 319, 230. Blick, M., et al. 1984. Blood 64, 1234. Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

References

Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
DiFiore, P.P., et al. 1987. Science 237, 178.
Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
Slamon, D.J., et al. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319, 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative control	Fisher rat embryo cells
Positive control	B104-1-1 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ200

Applications
Application References	Immunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Not for Neutralization Studies Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation Not Neutralization Studies
Application Notes	Frozen Sections (5 µg/ml, see application references) Immunoblotting (see application references) Immunocytochemistry (5 µg/ml) Immunoprecipitation (1 µg/ml, see application references) Neutralization Studies (not recommended, see application references)
Application Comments	Stains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	B104-1-1 cells overexpressing rat c-ErbB2/c-Neu
Immunogen	Human
Epitope	Within the extracellular domain
Clone	7.16.4
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP16-100UGCN	04055977208801

Documentation

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) MSDS

职位
物料安全数据表 (MSDS)

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) 分析证书

标题	批号
OP16	输入批号

参考

参考信息概述
Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15. Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239. DiFiore, P.P., et al. 1987. Science 237, 178. Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Slamon, D.J., et al. Science 235, 177. Varley, J.M., et al. 1987. Oncogene 1, 423. Bargmann, C.I., et al. 1986. Nature 319, 226. Yamamoto, T., et al. 1986. Nature 319, 230. Blick, M., et al. 1984. Blood 64, 1234. Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

参考信息概述

引用

标题

R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Synonyms	Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
Application	Frozen Sections (5 µg/ml, see application references) Immunoblotting (see application references) Immunocytochemistry (5 µg/ml) Immunoprecipitation (1 µg/ml, see application references) Neutralization Studies (not recommended, see application references)
Application Data	Detection of rat c-neu by immunocytochemistry. Sample: B104-1-1 cells overexpressing c-neu. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) (Cat. No. OP16) (1.4 µg/ml). Detection: fluorescence.
Description	Purified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-neu protein.
Background	Amplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
Host	Mouse
Immunogen species	Human
Immunogen	B104-1-1 cells overexpressing rat c-ErbB2/c-Neu
Epitope	Within the extracellular domain
Clone	7.16.4
Isotype	IgG_2a
Species	human, rat
Positive control	B104-1-1 cells
Negative control	Fisher rat embryo cells
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Stains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15. Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol. 17, 1031. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239. DiFiore, P.P., et al. 1987. Science 237, 178. Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Slamon, D.J., et al. Science 235, 177. Varley, J.M., et al. 1987. Oncogene 1, 423. Bargmann, C.I., et al. 1986. Nature 319, 226. Yamamoto, T., et al. 1986. Nature 319, 230. Blick, M., et al. 1984. Blood 64, 1234. Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Citation	R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
Application references	Immunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Not for Neutralization Studies Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.

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描述

OP16L

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)

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Life Science Research > Antibodies and Assays > Primary Antibodies

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