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OP14L
Sigma-AldrichAnti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)
Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.
More>>Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections. Less<<
Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Van De Vijver, M.J., et al.1988 New Engl. J. Med.319, 1239. DiFiore, P.P., et al.1987 Science237, 178. Slamon, D.J., et al. 1987 Science235, 177. Varley, J.M., et al.1987 Oncogene1, 423. Bargmann, C.I., et al. 1986 Nature319, 226. Yamamoto, T., et al.1986 Nature319, 230. Blick, M., et al. 1984 Blood64, 1234.
Immunoprecipitates the ~185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
cells overexpressing human c-ErbB2/c-Neu
Immunogen
Human
Clone
9G6
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Van De Vijver, M.J., et al.1988 New Engl. J. Med.319, 1239. DiFiore, P.P., et al.1987 Science237, 178. Slamon, D.J., et al. 1987 Science235, 177. Varley, J.M., et al.1987 Oncogene1, 423. Bargmann, C.I., et al. 1986 Nature319, 226. Yamamoto, T., et al.1986 Nature319, 230. Blick, M., et al. 1984 Blood64, 1234.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
Background
Amplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
Host
Mouse
Immunogen species
Human
Immunogen
cells overexpressing human c-ErbB2/c-Neu
Clone
9G6
Isotype
IgG₁
Species
human
Positive control
SK-BR-3 cells or breast carcinoma tissue
Negative control
HepG2 cells or normal skin
Form
Lyophilized
Formulation
Lyophilized from a volatile buffer, 100 µg BSA.
Preservative
None
Comments
Immunoprecipitates the ~185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Van De Vijver, M.J., et al.1988 New Engl. J. Med.319, 1239. DiFiore, P.P., et al.1987 Science237, 178. Slamon, D.J., et al. 1987 Science235, 177. Varley, J.M., et al.1987 Oncogene1, 423. Bargmann, C.I., et al. 1986 Nature319, 226. Yamamoto, T., et al.1986 Nature319, 230. Blick, M., et al. 1984 Blood64, 1234.
Application references
Paraffin Sections
Van De Vijver, M.J., et al. 1988. New Engl. J. Med.319, 1239.
