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OP14 Sigma-AldrichAnti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
Data Sheet

同义词: Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
Catalogue Number	OP14
Brand Family	Calbiochem®
Synonyms	Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu

References
References	Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239. DiFiore, P.P., et al.1987 Science 237, 178. Slamon, D.J., et al. 1987 Science 235, 177. Varley, J.M., et al.1987 Oncogene 1, 423. Bargmann, C.I., et al. 1986 Nature 319, 226. Yamamoto, T., et al.1986 Nature 319, 230. Blick, M., et al. 1984 Blood 64, 1234.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative control	Normal skin tissue or HepG2 cells
Positive control	SK-BR-3 cells or breast carcinoma tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Paraffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
Key Applications	Frozen Sections Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (5 µg/ml) Immunoprecipitation (1 µg/sample) Paraffin Sections (see application references)
Application Comments	Immunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	cells overexpressing human c-ErbB2/c-Neu
Immunogen	Human
Clone	9G6
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP14-100UGCN	04055977224863

Documentation

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) 分析证书

标题	批号
OP14	输入批号

参考

参考信息概述
Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239. DiFiore, P.P., et al.1987 Science 237, 178. Slamon, D.J., et al. 1987 Science 235, 177. Varley, J.M., et al.1987 Oncogene 1, 423. Bargmann, C.I., et al. 1986 Nature 319, 226. Yamamoto, T., et al.1986 Nature 319, 230. Blick, M., et al. 1984 Blood 64, 1234.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	29-June-2007 RFH
Synonyms	Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu
Application	Frozen Sections (5 µg/ml) Immunoprecipitation (1 µg/sample) Paraffin Sections (see application references)
Description	This clone expresses the kappa light chain isotype. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
Background	Amplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
Host	Mouse
Immunogen species	Human
Immunogen	cells overexpressing human c-ErbB2/c-Neu
Clone	9G6
Isotype	IgG₁
Species	human
Positive control	SK-BR-3 cells or breast carcinoma tissue
Negative control	Normal skin tissue or HepG2 cells
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Immunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239. DiFiore, P.P., et al.1987 Science 237, 178. Slamon, D.J., et al. 1987 Science 235, 177. Varley, J.M., et al.1987 Oncogene 1, 423. Bargmann, C.I., et al. 1986 Nature 319, 226. Yamamoto, T., et al.1986 Nature 319, 230. Blick, M., et al. 1984 Blood 64, 1234.
Application references	Paraffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.

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产品目录编号

描述

OP14L

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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