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MABE351 Sigma-AldrichAnti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IP, ICC, ChIP-seq, ChIP	M	Ascites	Monoclonal Antibody

Description
Catalogue Number	MABE351
Description	Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5
Alternate Names	Histone H3.1t H3/t H3t H3/g
Background Information	Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes, with access to DNA, potentially resulting in increased gene transcription. In fission yeast, acetylation of H3K4 is regulated by Gcn5 and the Mst2 complex, and is crucial to the activation of DNA damage checkpoint.

References

Product Information
Format	Ascites
Control	HeLa acid extract
Presentation	Mouse monoclonal IgG1 in ascites containing 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.
Key Applications	Western Blotting Immunoprecipitation Immunocytochemistry ChIP-seq Chromatin Immunoprecipitation (ChIP)
Application Notes	Immunoprecipitation Analysis: A representative lot was used to immunoprecipitate acetyl-Histone H3 (Lys4) in IP. Immunocytochemistry Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ICC. Chromatin Immunoprecipitation Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ChIP. Western Blot Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in WB.

Biological Information
Immunogen	Ovalbumin-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys14.
Clone	clone 13HH3-1A5
Host	Mouse
Isotype	IgG1
Species Reactivity	Human Mouse
Species Reactivity Note	Demonstrated to react with Human. Demonstrated to react in Mouse ESCs in ChIP-seq in Karmodiya et al. BMC Genomics 2012, 13:424.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_003484
Gene Symbol	HIST3H3 H3FT
Purification Method	Unpurified
UniProt Number	Q16695
Molecular Weight	~17 kDa observed. Uncharacterized bands may be observed at ~23 kDa and ~35 kDa in some cell lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H3 (Lys4) in 10 µg of HeLa acid extract.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE351	04053252697760

Documentation

Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 MSDS

职位
物料安全数据表 (MSDS)

Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 分析证书

标题	批号
Anti-acetyl-Histone H3 (Lys14), -2520566	2520566
Anti-acetyl-Histone H3 (Lys14), -2665808	2665808
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5	3013345
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 2298448	2298448
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 3508428	3508428
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 3853802	3853802
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 4204627	4204627
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - Q2029943	Q2029943
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 -2705156	2705156
Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 Monoclonal Antibody	2959567

参考

参考概述	应用	品种	公共医疗ID
H3K9 and H3K14 acetylation co-occur at many gene regulatory elements, while H3K14ac marks a subset of inactive inducible promoters in mouse embryonic stem cells. Karmodiya, Krishanpal, et al. BMC Genomics, 13: 424 (2012) 2012 显示摘要 ABSTRACT:	Chromatin Immunoprecipitation (ChIP)	Mouse	22920947

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