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MABT339 Sigma-AldrichAnti-ZO-1 Antibody, clone 5G6.1

This Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .

Anti-ZO-1 Antibody, clone 5G6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: TJP1, ZO1 UniProt Number: Q07157

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB, IH(P)	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABT339
Description	Anti-ZO-1 Antibody, clone 5G6.1
Alternate Names	Tight junction protein ZO-1 Tight junction protein 1 Zona occludens protein 1 Zonula occludens protein 1 ZO-1
Background Information	Tight junction protein ZO-1 (UniProt Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 gene (also known as ZO1) (Gene ID 7082) in human. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, ZO-2, and ZO-3. ZO-1, a 210-225 kDa protein, is found at the submembranous domain of TJs in epithelia and endothelia. ZO-1 contains three PDZ domains (PDZ1/aa23-109; PDZ2/aa181-261; PDZ3/aa422-502) at its N-treminal end, followed by an SH3 domain (aa519-580), a GUK/GK homology domain (aa632-782), an acidic domain (aa817-894), an alpha spliced domain (aa921-1000), and a C-terminal proline-rich/PR domain. ZO-1 is a phosphoprotein and a known substrate of serine/threonine kinases ZAK and of PKC. MAPK signaling pathway regulates tyrosine phosphorylation of ZO-1, and MEK1 inhibition in Ras transformed epithelial cells is reported to result in tyrosine phosphorylation of ZO-1 and occludin. ZO-1 interacts with claudins, JAM, ZO-2, and ZO-3 through its PDZ domains, while its GK module mediates interaction with occludin. ZO-1 also binds actin cytoskeleton and actin-binding protein 4.1 through its carboxyl terminal end. In addition, ZO-1 associates with AF-6 and cingulin at the TJ, as well as with the adherens junction protein alpha-catenin and with the gap junction proteins connexins 43 and 45.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in human and rat kidney tissues.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human ZO-1.
Clone	5G6.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG2aκ
Species Reactivity	Human Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_003248
Gene Symbol	TJP1 ZO1
Purification Method	Protein G Purified
UniProt Number	Q07157
Molecular Weight	~195 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HCT116 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 200 µg of HCT116 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABT339	04055977173505

Documentation

Anti-ZO-1 Antibody, clone 5G6.1 MSDS

职位
物料安全数据表 (MSDS)

Anti-ZO-1 Antibody, clone 5G6.1 分析证书

标题	批号
Anti-ZO-1, clone 5G6.1 - 3219908	3219908
Anti-ZO-1, clone 5G6.1 - 3274285	3274285
Anti-ZO-1, clone 5G6.1 - 3590146	3590146
Anti-ZO-1, clone 5G6.1 - 3744303	3744303
Anti-ZO-1, clone 5G6.1 - 3891833	3891833
Anti-ZO-1, clone 5G6.1 - 3923541	3923541
Anti-ZO-1, clone 5G6.1 - 3958817	3958817
Anti-ZO-1, clone 5G6.1 - 3988493	3988493
Anti-ZO-1, clone 5G6.1 - 4023307	4023307
Anti-ZO-1, clone 5G6.1 - 4044216	4044216

技术信息

标题
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

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描述

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Anti-ZO-1 Antibody

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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