Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
04-November-2010 RFH
Synonyms
Anti-X-ray Repair Complementing Defective Repair in CHO1
Application
Immunoblotting (1-5 µg/ml)
Application Data
Detection of mouse XRCC1 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-XRCC1 Rabbit pAb (Cat. No. DR1103) (1-5 and micro;g/ml). Detection: chemiluminescence.
Description
Purified rabbit polyclonal antibody. Recognizes the ~75 kDa XRCC1 protein.
Background
The protein is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells.
Host
Rabbit
Immunogen species
Human
Immunogen
Full-length, human XRCC1 (aa 1-633)
Isotype
IgG
Species
human, mouse
Positive control
Mouse kidney tissue
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
