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MABF2234-25UG Anti-West Nile Virus Antibody, clone E16

MABF2234-25UG
25 μg  
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      概述

      Replacement Information
      Description
      Catalogue NumberMABF2234-25UG
      DescriptionAnti-West Nile Virus Antibody, clone E16
      Alternate Names
      • E protein
      • Envelope protein
      • Env
      Background InformationWest Nile virus (WNV) is a mosquito-borne positive-sense RNA virus of the Flavivirus genus within the Flaviviridae family. WNV genome encodes for three structural domains known as C, prM, and E and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). These are translated as a single polypeptide, which is subsequently cleaved by viral and cellular proteases. The envelope glycoprotein (E) undergoes a conformational rearrangement triggered by low pH that results in a class II fusion event required for viral entry. The E protein contains a three-domain structure where domain I is an eight-stranded -barrel in the center portion of the protein (aa 1-51, 134-195, and 284-297). The DII domain consists of 170 residues inserted into DI (aa 52-133 and 196-283). At the distal end of DII domain is a fusion loop (aa 98-110) and a conserved glycine-rich, hydrophobic sequence, which is important for flaviviral fusion. The third domain, DIII, is an Ig-like domain with seven -strand and has been implicated in receptor binding. The E protein also has a single N-linked glycosylation site at Asn 154, located within the ectodomain of prM domain and is cleaved by cellular enzymes during viral particle maturation. Clone E16 specifically binds to the DIII lateral ridge and displays high neutralizing activity and is reported to completely inhibit infection even at 10 ng/mL. (Ref.: Nybakken, GE et al. (2006). J. Virol. 80(23); 11467-11474; Oliphant, T., et al. (2006). J. Virol. 80(24); 12149-12159).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
      Applications
      ApplicationAnti-West Nile Virus, clone E16, Cat. No. MABF2234, is a highly specific mouse monoclonal antibody that targets WNV envelope protein and has been tested for use in Immunocytochemistry, Neutralizing Applications, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Neutralizing
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected West Nile Virus in Immunocytochemistry applications (Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).

      Neutralizing Analysis: A representative lot completely inhibited West Nile virus infection at 10 ng/mL. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).
      Biological Information
      ImmunogenWest Nile Virus envelope protein.
      CloneE16
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone E16 is a mouse monoclonal antibody that specifically detects envelope protein of West Nile Virus. It targets an epitope within the DIII lateral ridge region.
      IsotypeIgG2bκ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • E
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 31.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with Full-length ectodomain of West Nile Virus envelope protein.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Full-length ectodomain of West Nile Virus envelope protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MABF2234-25UG 04061841039112

      Documentation

      Anti-West Nile Virus Antibody, clone E16 MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-West Nile Virus Antibody, clone E16 分析证书

      标题批号
      Anti-West Nile Virus, clone E16 - 3277845 3277845

      参考

      参考概述公共医疗ID
      A therapeutic antibody against west nile virus neutralizes infection by blocking fusion within endosomes.
      Thompson, BS; Moesker, B; Smit, JM; Wilschut, J; Diamond, MS; Fremont, DH
      PLoS Pathog  5  e1000453  2009

      显示摘要
      19478866 19478866
      Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein.
      Oliphant, T; Nybakken, GE; Engle, M; Xu, Q; Nelson, CA; Sukupolvi-Petty, S; Marri, A; Lachmi, BE; Olshevsky, U; Fremont, DH; Pierson, TC; Diamond, MS
      J Virol  80  12149-59  2006

      显示摘要
      17035317 17035317