数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

GR37 Sigma-AldrichAnti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)

This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Am, Ch, Ht, H, M, Porcine, R	R	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.
Catalogue Number	GR37
Brand Family	Calbiochem®

References
References	Brown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11. Fleet, J.C. 1999. Nutr. Rev. 57 60. Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591. Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335. Issa, L.L., et al. 1998. Inflamm. Res. 47, 451. Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121. Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.

References

Brown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11.
Fleet, J.C. 1999. Nutr. Rev. 57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res. 47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.

Product Information
Form	Liquid
Formulation	In 10 mM PBS, 0.2% BSA, pH 7.4.
Positive control	Breast carcinoma tissue (see comments)
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Gel Shift Immunoblotting (Western Blotting) Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (1:50-1:100) Gel Shift (see comments) Immunoblotting (1:200-1:400) Immunoprecipitation (10 µl/mg protein lysate) Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Application Comments	Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	purified, chick intestine vitamin D receptor
Immunogen	Chicken
Clone	9A7γE10.4
Host	Rat
Isotype	IgG₁
Species Reactivity	Amphibian Chicken Hamster Human Mouse Porcine Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
GR37-100UGCN	04055977228311

Documentation

Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) MSDS

职位
物料安全数据表 (MSDS)

Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) 分析证书

标题	批号
GR37	输入批号

参考

参考信息概述
Brown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11. Fleet, J.C. 1999. Nutr. Rev. 57 60. Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591. Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335. Issa, L.L., et al. 1998. Inflamm. Res. 47, 451. Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121. Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.

参考信息概述

引用

标题

Roberto Paredes, et al. (2004) Bone-Specific Transcription Factor Runx2 Interacts with the 1α,25-Dihydroxyvitamin D3 Receptor To Up-Regulate Rat Osteocalcin Gene Expression in Osteoblastic Cells. Molecular and Cellular Biology 24, 8847-8861.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	27-August-2007 RFH
Application	Frozen Sections (1:50-1:100) Gel Shift (see comments) Immunoblotting (1:200-1:400) Immunoprecipitation (10 µl/mg protein lysate) Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Description	Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
Background	Vitamin D Receptor (VDR) also known as 1,25-dihydroxyvitamin D3 receptor is a member of the nuclear steroid hormone receptor superfamily which regulate gene transcription by interacting with response elements in gene promoters. VDR binds with the retinoid X receptor (RXR) from specific DNA sequences in the promoter region of vitamin D response elements (VDREs). VDR has been implicated in a variety of physiological functions including effects on growth and differentiation in normal and malignant cells. These effects are mediated through a single ligand, calcitriol. Cacitriol the active metabolite of vitamin D is a known regulator of calcium homeostasis but it also has numerous other functions including inhibition of proliferation of cancer cells and suppression of T-cell proliferation and cytokine production. Human breast carcinomas, leukemias, and prostatic carcinoma cells all express vitamin D receptor, suggesting that vitamin D may have beneficial actions on various malignancies that may prove useful in chemoprevention and/or differentiation therapy.
Host	Rat
Immunogen species	Chicken
Immunogen	purified, chick intestine vitamin D receptor
Clone	9A7γE10.4
Isotype	IgG₁
Species	amphibian, chicken, hamster, human, mouse, porcine, rat
Positive control	Breast carcinoma tissue (see comments)
Form	Liquid
Formulation	In 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw +2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial use, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Brown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11. Fleet, J.C. 1999. Nutr. Rev. 57 60. Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591. Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335. Issa, L.L., et al. 1998. Inflamm. Res. 47, 451. Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121. Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.
Citation	Roberto Paredes, et al. (2004) Bone-Specific Transcription Factor Runx2 Interacts with the 1α,25-Dihydroxyvitamin D3 Receptor To Up-Regulate Rat Osteocalcin Gene Expression in Osteoblastic Cells. Molecular and Cellular Biology 24, 8847-8861.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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