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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

CP74 Sigma-AldrichAnti-Vinculin Mouse mAb (V284 or VLN01)

This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.

Anti-Vinculin Mouse mAb (V284 or VLN01) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
Catalogue Number	CP74
Brand Family	Calbiochem®
Application Data	Detection of human vinculin by immunoblotting. Sample: Human vinculin. Primary antibody: Anti-Vinculin Mouse mAb (V284 or VLN01) (Cat. No. CP74) (1 µg/ml). Detection: chemiluminescence.

References
References	Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961. Bakolitsa, C., et al. 1999 Cell 99, 603. Rudiger, M., et al. 1998 Bioessays 20, 733 Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155 Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1. Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131 Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.

References

Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
Bakolitsa, C., et al. 1999 Cell 99, 603.
Rudiger, M., et al. 1998 Bioessays 20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.

Product Information
Form	Liquid
Formulation	In 10 mM PBS, 0.2% BSA, pH 7.4.
Negative control	Normal mouse serum
Positive control	Skin cells or tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunoprecipitation Paraffin Sections
Application Notes	Immunoblotting (1 µg/ml, chemiluminescence) Immunoprecipitation (2 µg/ml lysate) Paraffin Sections (4-8 µg/ml, heat pre-treatment required)
Application Comments	This antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	semi-purified, human vinculin
Immunogen	Human
Clone	V284 or VLN01
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
CP74-100UGCN	04055977221022

Documentation

Anti-Vinculin Mouse mAb (V284 or VLN01) MSDS

职位
物料安全数据表 (MSDS)

Anti-Vinculin Mouse mAb (V284 or VLN01) 分析证书

标题	批号
CP74	输入批号

参考

参考信息概述
Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961. Bakolitsa, C., et al. 1999 Cell 99, 603. Rudiger, M., et al. 1998 Bioessays 20, 733 Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155 Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1. Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131 Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	22-August-2007 RFH
Application	Immunoblotting (1 µg/ml, chemiluminescence) Immunoprecipitation (2 µg/ml lysate) Paraffin Sections (4-8 µg/ml, heat pre-treatment required)
Application Data	Detection of human vinculin by immunoblotting. Sample: Human vinculin. Primary antibody: Anti-Vinculin Mouse mAb (V284 or VLN01) (Cat. No. CP74) (1 µg/ml). Detection: chemiluminescence.
Description	Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.
Background	Vinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and α-actinin, which in turn binds to actin. Also, vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor. Vinculin has been associated with tumor suppressor activity. Vinculin is widely distributed in tissues and expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix. Vinculin has been found in all adherens junctions, while the larger splice variant, meta-vinculin, is found only in smooth and cardiac muscle.
Host	Mouse
Immunogen species	Human
Immunogen	semi-purified, human vinculin
Clone	V284 or VLN01
Isotype	IgG₁
Species	human
Positive control	Skin cells or tissue
Negative control	Normal mouse serum
Form	Liquid
Formulation	In 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	This antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961. Bakolitsa, C., et al. 1999 Cell 99, 603. Rudiger, M., et al. 1998 Bioessays 20, 733 Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155 Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1. Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131 Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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