Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
Clone
4A8
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-November-2010 RFH
Synonyms
Anti-Valosin-Containing Protein
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Data
Detection of human VCP by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (1-5 µg/ml). Detection: chemiluminescence. Detection of human VCP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (3 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
Background
VCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
Host
Mouse
Immunogen
Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
Clone
4A8
Isotype
IgG2a
Species
human
Positive control
HePG2 cells, human placenta tissue
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
