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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ST1200 Sigma-AldrichAnti-Ubiquitin Mouse mAb (FK2)

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.

Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
A Broad Range Of Species	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
Catalogue Number	ST1200
Brand Family	Calbiochem®
Application Data	Detection of ubiquitin by immunoblotting. Samples: K⁴⁸-linked ubiquitin chains (lanes A and D), K²⁹-linked ubiquitin chains (lanes B and E), and K⁶³-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.

References
References	Matsumoto, M., et al. 2005. Proteomics 5, 4545. Boname, J.N., et al. 2001. Immunity 15, 627. Campbell, V., et al. 2001. Neuron 32, 1013. Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.

Product Information
Form	Liquid
Formulation	In PBS.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	ELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation
Application Notes	ELISA (see application references) Immunoblotting (1:1000) Immunoprecipitation (see application references and comments) Immunofluorescence (see application references)
Application Comments	Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	unpurified polyubiquitinylated lysozyme
Immunogen	human
Clone	FK2
Host	Mouse
Isotype	IgG₁
Source	Raji cells
Species Reactivity	A Broad Range Of Species
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ST1200-100UGCN	04055977224078

Documentation

Anti-Ubiquitin Mouse mAb (FK2) MSDS

职位
物料安全数据表 (MSDS)

Anti-Ubiquitin Mouse mAb (FK2) 分析证书

标题	批号
ST1200	输入批号

参考

参考信息概述
Matsumoto, M., et al. 2005. Proteomics 5, 4545. Boname, J.N., et al. 2001. Immunity 15, 627. Campbell, V., et al. 2001. Neuron 32, 1013. Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-March-2009 JSW
Application	ELISA (see application references) Immunoblotting (1:1000) Immunoprecipitation (see application references and comments) Immunofluorescence (see application references)
Application Data	Detection of ubiquitin by immunoblotting. Samples: K⁴⁸-linked ubiquitin chains (lanes A and D), K²⁹-linked ubiquitin chains (lanes B and E), and K⁶³-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
Description	Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins.
Host	Mouse
Immunogen species	human
Immunogen	unpurified polyubiquitinylated lysozyme
Clone	FK2
Isotype	IgG₁
Species	a broad range of species
Form	Liquid
Formulation	In PBS.
Concentration Label	Please refer to vial label for lot-specific concentration
Source	Raji cells
Preservative	≤0.1% sodium azide
Comments	Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Toxicity	Standard Handling
References	Matsumoto, M., et al. 2005. Proteomics 5, 4545. Boname, J.N., et al. 2001. Immunity 15, 627. Campbell, V., et al. 2001. Neuron 32, 1013. Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Application references	ELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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