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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

05-1946 Sigma-AldrichAnti-USP7 Antibody, clone 1G7

This Anti-USP7 Antibody, clone 1G7 is validated for use in WB, IP, IH for the detection of USP7.

Anti-USP7 Antibody, clone 1G7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: USP7, TEF1, HAUSP UniProt Number: Q93009

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概述

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IP, IHC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-1946
Description	Anti-USP7 Antibody, clone 1G7
Alternate Names	ubiquitin specific peptidase 7 (herpes virus-associated) Ubiquitin-specific-processing protease 7 ubiquitin specific protease 7 (herpes virus-associated) Herpesvirus-associated ubiquitin-specific protease Herpes virus-associated ubiquitin-specific protease Ubiquitin thioesterase 7 ubiquitin specific peptidase 7 Deubiquitinating enzyme 7 HAUSP
Background Information	Ubiquitin carboxyl-terminal hydrolase 7 (USP7) is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates.[Saridakis, 2005] Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, Ubiquitin carboxyl-terminal hydrolase 7 activity generally stabilizes its substrate proteins. Ubiquitin carboxyl-terminal hydrolase 7 is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.[Canning, 2004]

References

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-USP7 Antibody, clone 1G7 is validated for use in WB, IP, IH for the detection of USP7.
Key Applications	Western Blotting Immunoprecipitation Immunohistochemistry

Biological Information
Immunogen	Histadine-tagged recombinant protein corresponding to human USP7.
Clone	1G7
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes USP7.
Isotype	IgG1κ
Species Reactivity	Human Mouse
Species Reactivity Note	Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_003461.2
Gene Symbol	USP7 TEF1 HAUSP
Purification Method	Protein G Purified
UniProt Number	Q93009
UniProt Summary	FUNCITON: Cleaves ubiquitin fusion protein substrates. Deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis. CATALYTIC ACTIVITY: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. ENZYME REGULATION: Inhibited by N-ethyl-maleimide (NEM) and divalent cations. Tolerates high concentrations of NaCl but is inhibited at concentrations of 195 mM and higher. Ref.7 SUBUNIT STRUCTURE: Monomer. Interacts with TP53, MDM2 and UBXN6. Interacts with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1. EBNA1 shows a 10-fold higher affinity than TP53 and can compete with it for USP7 binding. Binding to VMW110 may modulate the substrate specificity or activity of USP7 to stabilize viral proteins. Interacts with ATXN1 and the strength of interaction is influenced by the length of the poly-Gln region in ATXN1. A weaker interaction seen with mutants having longer poly-Gln regions. SUBCELLULAR LOCATION: Nucleus. Note: Present in a minority of ND10 nuclear bodies. Association with VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. TISSUE SPECIFICTY: Widely expressed. PTM: Polyneddylated (By similarity). Polyubiquitinated (By similarity). SEQUENCE SIMILARITIES: Belongs to the peptidase C19 family. Contains 1 MATH domain. BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Active from pH 7.0 to 9.5.
Molecular Weight	~ 128kDa Observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected USP7 on 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
05-1946	04053252628818

Documentation

Anti-USP7 Antibody, clone 1G7 MSDS

职位
物料安全数据表 (MSDS)

Anti-USP7 Antibody, clone 1G7 分析证书

标题	批号
Anti-USP7, clone 1G7	3049391
Anti-USP7, clone 1G7 - 2142201	2142201
Anti-USP7, clone 1G7 - 2145101	2145101
Anti-USP7, clone 1G7 - 2167179	2167179
Anti-USP7, clone 1G7 - 2436808	2436808
Anti-USP7, clone 1G7 - 2227186	2227186
Anti-USP7, clone 1G7 - 3215514	3215514
Anti-USP7, clone 1G7 - 3394228	3394228
Anti-USP7, clone 1G7 - 3588023	3588023
Anti-USP7, clone 1G7 - 3836575	3836575

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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