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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABN814 Sigma-AldrichAnti-Twinkle Antibody, clone 13A10.1

This Anti-Twinkle Antibody, clone 13A10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Twinkle.

Anti-Twinkle Antibody, clone 13A10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: EPO1, C10orf2 UniProt Number: Q96RR1

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IH(P)	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN814
Description	Anti-Twinkle Antibody, clone 13A10.1
Alternate Names	Twinkle protein, mitochondrial Progressive external ophthalmoplegia 1 protein T7 gp4-like protein with intramitochondrial nucleoid localization T7-like mitochondrial DNA helicase Twinkle
Background Information	Twinkle protein, mitochondrial (EC 3.6.4.12; UniProt Q96RR1; also known as Ataxin 8, Mitochondrial twinkle protein, Progressive external ophthalmoplegia 1 protein, T7 gp4-like protein with intramitochondrial nucleoid localization, T7 helicase-related protein with intramitochondrial nucleoid localization, T7-like mitochondrial DNA helicase) is encoded by the EPO1 (also known as ATXN8, C10orf2, IOSCA, MTDPS7, PEO, PEO1, PEOA3, PRLTS5, SANDO, SCA8, TWINL) gene (Gene ID 56662) in human. Mitochondrial DNA (mtDNA) replication involves the catalytic and accessory subunits of DNA polymerase gamma, the mtDNA helicase Twinkle, the mitochondrial single-stranded DNA-binding protein, and the mitochondrial RNA polymerase. Cells expressing Twinkle mutants accumulate double-stranded mtDNA replication intermediates with loss of RNA associated with the lagging strand, consistent with the role of Twinkle in unwinding double-stranded mtDNA. Mutations in the human Twinkle gene (C10orf2) cause autosomal dominant progressive external ophthalmoplegia (PEO), associated with multiple mtDNA deletions.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Twinkle Antibody, clone 13A10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Twinkle.
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Twinkle in 10 µg of K562 cell lysate. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Twinkle in human cerebral cortex and human skeletal muscle tissue.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the C-terminal of human Twinkle.
Epitope	C-terminal
Clone	13A10.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG2aκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_068602
Gene Symbol	EPO1 C10orf2
Purification Method	Protein G Purified
UniProt Number	Q96RR1
Molecular Weight	~77 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HL-60 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Twinkle in 10 µg of HL-60 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN814	04055977274967

Documentation

Anti-Twinkle Antibody, clone 13A10.1 MSDS

职位
物料安全数据表 (MSDS)

Anti-Twinkle Antibody, clone 13A10.1 分析证书

标题	批号
Anti-Twinkle, clone 13A10.1 - 3446267	3446267
Anti-Twinkle, clone 13A10.1 - 3873554	3873554
Anti-Twinkle, clone 13A10.1 - 3992934	3992934
Anti-Twinkle, clone 13A10.1 -Q2592793	Q2592793

技术信息

标题
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White Paper: Further considerations of antibody validation and usage.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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