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MABT1337-25UG Anti-Tropomyosin alpha-1 Antibody, clone 2F4E11

Anti-Tropomyosin alpha-1, clone 2F4E11 , Cat. No. MABT1337, is a mouse monoclonal antibody that detects Tropomyosin alpha-1 chain and is tested for use in Western Blotting.

MABT1337-25UG
25 μg  
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      概述

      Replacement Information
      Description
      Catalogue NumberMABT1337-25UG
      DescriptionAnti-Tropomyosin alpha-1 Antibody, clone 2F4E11
      Alternate Names
      • Tropomyosin alpha-1 chain
      • Alpha-tropomyosin
      • Tropomyosin-1
      Background InformationTropomyosin alpha-1 chain (UniProt: P04692; also known as Alpha-tropomyosin, Tropomyosin-1) is encoded by the Tpm1 (also known as Alpha-tm, Tpma) gene (Gene ID: 24851) in rat. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Tropomyosin-1 is a cytoskeletal protein that associates with F-actin stress fibers. It binds to actin filaments in muscle and non-muscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It stabilizes actin filaments and regulates the interaction of actin filament with other actin-binding proteins. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Tropomyosin-1 is a homodimeric protein, but can form heterodimer of an alpha (TPM1, TPM3, or TPM4) and a beta (TPM2) chain. Mutations in TPM1 gene have been linked to cardiomyopathy characterized by ventricular hypertrophy and ventricular dilation and impaired systolic function that results in congestive heart failure and arrhythmia. Eight different isoforms of Tropomyosin-1 have been described in rat that are produced by alternative splicing. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Applications
      ApplicationAnti-Tropomyosin alpha-1, clone 2F4E11 , Cat. No. MABT1337, is a mouse monoclonal antibody that detects Tropomyosin alpha-1 chain and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Tropomyosin alpha-1in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of rat Tropomyosin alpha-1 chain, isoform 4.
      Clone2F4E11
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2F4E11 is a mouse monoclonal antibody that detects rat Tropomyosin alpha-1 chain. It targets an epitope within 19 amino acids from the C-terminal region corresponding to exon 9b in TPM1.11 gene.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TPM1
      • C15orf13
      • TMSA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~31 kDa observed; 28.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Untransfected parental rat B35 cells (neg ctrl) versus rat B35 cells transfected with Tpm1.1(pos ctrl).

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Tropomyosin alpha-1 in Untransfected parental rat B35 cells (neg ctrl) versus rat B35 cells transfected with Tpm1.1(pos ctrl).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MABT1337-25UG 04061841844587

      Documentation

      Anti-Tropomyosin alpha-1 Antibody, clone 2F4E11 MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-Tropomyosin alpha-1 Antibody, clone 2F4E11 分析证书

      标题批号
      Anti-Tropomyosin alpha-1, clone 2F4E11 - Q3437402 Q3437402

      参考

      参考概述公共医疗ID
      Tropomyosin isoforms and reagents
      Galina Schevzov 1 , Shane P Whittaker, Thomas Fath, Jim Jc Lin, Peter W Gunning
      Bioarchitecture  1(4)  135-164  2011

      显示摘要       		22069507 22069507