It appears that your browser has JavaScript disabled.

This Website requires your browser to be JavaScript enabled.

Please enable JavaScript and reload this page.

MAB10762 Sigma-AldrichAnti-Transthyretin Antibody, clone 7G8-E11-E7

This Anti-Transthyretin Antibody, clone 7G8-E11-E7 is validated for use in ELISA, WB for the detection of Transthyretin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases, Neurotransmitters & Receptors Gene Symbol: TTR, ATTR, PALB, Prealbumin, TBPA, transthyretin UniProt Number: P02766

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ELISA, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB10762
Description	Anti-Transthyretin Antibody, clone 7G8-E11-E7
Alternate Names	prealbumin, amyloidosis type I thyroxine-binding prealbumin TBPA TTR ATTR
Background Information	Transthyretin is one of the three major thyroid hormone-binding proteins in plasma and/or cerebrospinal fluid of vertebrates. It exists mainly as a homotetrameric protein of approximately 55 kDa in plasma and, in sera and cerebrospinal fluid, is a carrier of the thyroid hormone thyroxine (T4) and retinol. While mostly known for being the transporter of thyroxine and retinol, when mutated, TTR is also well-described as the cause of familial amyloid polyneuropathy, a neurodegenerative lethal disorder characterized by systemic deposition of TTR amyloid fibrils, particularly in the peripheral nervous system. Recently, studies have shown that TTR has an important function development of peripheral and central nervous system physiology, namely by participating in behavior, in the maintenance of normal cognitive processes during ageing, amidated neuropeptide processing and nerve regeneration. Additionally, it has been proposed that TTR is neuroprotective in Alzheimer's disease, by preventing the formation of amyloid beta fibrils (Fleming C.E. et al. (2009) Prog Neurobiol., Epub ahead of print

References

Product Information
Format	Purified
Control	Human placenta lysate
Presentation	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Transthyretin Antibody, clone 7G8-E11-E7 is validated for use in ELISA, WB for the detection of Transthyretin.
Key Applications	ELISA Western Blotting

Biological Information
Immunogen	Purified human Transthyretin.
Epitope	Unknown
Clone	7G8-E11-E7
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes Transthyretin.
Isotype	IgG2aκ
Species Reactivity	Human
Species Reactivity Note	Proven to react with human.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000362.1
Entrez Gene Summary	This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq]
Gene Symbol	TTR ATTR PALB Prealbumin TBPA transthyretin
Purification Method	Protein G Purified
UniProt Number	P02766
UniProt Summary	FUNCTION: Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. SUBUNIT STRUCTURE: Homotetramer. Dimer of dimers. In the homotetramer, subunits assemble around a central channel that can accomodate two ligand molecules. Interacts with RBP4. SUBCELLULAR LOCATION: Secreted. Cytoplasm. TISSUE SPECIFICITY: Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver. DOMAIN: Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel. INVOLVEMENT IN DISEASE: Defects in TTR are the cause of amyloidosis type 1 (AMYL1) [MIM:176300]. AMYL1 is a hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. Defects in TTR are the cause of amyloidosis type 7 (AMYL7) [MIM:105210]; also known as leptomeningeal amyloidosis or meningocerebrovascular amyloidosis. AMYL7 is a form of hereditary transthyretin amyloidosis characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor. Mild systemic amyloidosis may occurr. Defects in TTR are a cause of hyperthyroxinemia [MIM:176300]. MISCELLANEOUS: Tetramer dissociation and partial unfolding leads to the formation of aggregates and amyloid fibrils. Small molecules that occupy at least one of the thyroid hormone binding sites stabilize the tetramer, and thereby stabilize the native state and protect against misfolding and the formation of amyloid fibrils. Two binding sites for thyroxine are located in the channel. Less than 1% of plasma prealbumin molecules are normally involved in thyroxine transport. L-thyroxine binds to the transthyretin by an order of magnitude stronger than does the triiodo-L-thyronine. Thyroxine-binding globulin is the major carrier protein for thyroid hormones in man. About 40% of plasma transthyretin circulates in a tight protein-protein complex with the plasma retinol-binding protein (RBP). The formation of the complex with RBP stabilizes the binding of retinol to RBP and decreases the glomerular filtration and renal catabolism of the relatively small RBP molecule. There is evidence for 2 binding sites for RBP, one possibly being a region that includes Ile-104, located on the outer surface of the transthyretin molecule. SEQUENCE SIMILARITIES: Belongs to the transthyretin family.
Molecular Weight	Transthyretin exists mainly as a homotetrameric protein of approximately 55 kDa in plasma. A single band of ~26 kDa was observed, which is an approximate size of a dimeric component that makes up the said homotetramer.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human placenta lysate. Western Blot Analysis: 2 µg/ml of this antibody detected Transthyretin in 10 µg of human placenta lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MAB10762	04053252399657

Documentation

Anti-Transthyretin Antibody, clone 7G8-E11-E7 MSDS

职位
物料安全数据表 (MSDS)

Anti-Transthyretin Antibody, clone 7G8-E11-E7 分析证书

标题	批号
Anti-Transthyretin, clone 7G8-E11-E7 - 2377297	2377297
Anti-Transthyretin, clone 7G8-E11-E7 - 2424102	2424102
Anti-Transthyretin, clone 7G8-E11-E7 - 2210366	2210366
Anti-Transthyretin, clone 7G8-E11-E7 - 3211115	3211115
Anti-Transthyretin, clone 7G8-E11-E7 - 3934984	3934984
Anti-Transthyretin, clone 7G8-E11-E7 - 4091261	4091261
Anti-Transthyretin, clone 7G8-E11-E7 - NG1753058	NG1753058
Anti-Transthyretin, clone 7G8-E11-E7 - NRG1665730	NRG1665730
Anti-Transthyretin, clone 7G8-E11-E7 -2743630	2743630
Anti-Transthyretin, clone 7G8-E11-E7 -2776278	2776278

相关产品和应用

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

最近查看的内容