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GR08L
Sigma-AldrichAnti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14)
This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1).
More>>This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor in the plasma membrane in proliferating cells.
Catalogue Number
GR08L
Brand Family
Calbiochem®
Synonyms
Anti-TR, Anti-CD71
References
References
Schneider, C., et al., 1982. J. Biol. Chem.257, 8516. Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175. Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem.49, 357. Omary, M.B., et al. 1980. Nature286, 888. Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.
Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
K562 cells
Immunogen
Human
Clone
T56/14
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Global Trade Item Number
产品目录编号
GTIN
GR08L-100UGCN
04055977228199
Documentation
Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) MSDS
Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) 分析证书
标题
批号
GR08L
参考
参考信息概述
Schneider, C., et al., 1982. J. Biol. Chem.257, 8516. Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175. Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem.49, 357. Omary, M.B., et al. 1980. Nature286, 888. Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor protein.
Background
Transferrin is the major iron binding protein in human plasma and is bound by a specific cell surface receptor during transport of iron into various tissues. The human transferrin receptor was originally isolated from placenta and shown to contain a subunit with an apparent molecular weight of 90,000. Further structural characterization of the receptor revealed that it resides in the plasma membrane as a 180,000 dimer, has a pI of 5.2, is phosphorylated predominantly on serine residues and contains both complex and high mannose oligosaccharide chains. Transferrin receptor is considered to be a marker for proliferating cells and its levels can be drastically reduced by agents which cause differentiation of undifferentiated cells such as HL-60 cells. Since many of the enzymes of metabolically active, proliferating cells require iron to catalyze redox reactions it is not surprising that the transferrin receptor would be a marker of proliferation. Further support for this hypothesis has been obtained by using antibodies to block transferrin binding and cause the accumulation of cells in S-phase, presumably due to interference with normal iron uptake and energy metabolism. Thus, monoclonal antibodies recognizing the transferrin receptor are not only valuable tools for studying iron metabolism, but may also be useful for monitoring and possible therapy of diseases involving highly proliferative cells.
Host
Mouse
Immunogen species
Human
Immunogen
K562 cells
Clone
T56/14
Isotype
IgG₁
Species
human
Positive control
Most cell lines or placental tissue
Form
Lyophilized
Formulation
Lyophilized from a volatile buffer, 100 µg BSA.
Preservative
None
Comments
Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Schneider, C., et al., 1982. J. Biol. Chem.257, 8516. Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175. Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem.49, 357. Omary, M.B., et al. 1980. Nature286, 888. Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.
Application references
Original Clone
Trowbridge, I.S. and Omary, M.B., 1981. Proc. Natl. Acad. Sci. USA 3039.
