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MABS1273 Sigma-AldrichAnti-Thrombomodulin Antibody, clone 411-201B

Anti-Thrombomodulin Antibody, clone 411-201B is an antibody against Thrombomodulin for use in Immunohistochemistry (Paraffin), Electron Microscopy, Immunoprecipitation, Western Blotting, ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Thbd UniProt Number: P15306

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H	IH(P), EM, IP, WB, ELISA	R	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS1273
Description	Anti-Thrombomodulin Antibody, clone 411-201B
Alternate Names	Thrombomodulin CD141 Fetomodulin SnoRNA MBII-339 TM
Background Information	Thrombomodulin (UniProt P15306; also known as CD141, Fetomodulin, SnoRNA MBII-339, TM) is encoded by the Thbd gene (Gene ID 21824) in murine species. Thrombomodulin (TM) is an endothelial cell surface protein that functions to suppress the propagation of thrombin (activated blood-coagulation factor II) generation by acting as a thrombin receptor. Formation of the TM-thrombin complex abolishes thrombin's procoagulant activity by altering its substrate specificity, leading to proteolytic cleavage of protein C into the natural anticoagulant termed "activated protein C". Together with protein S, activated protein C proteolytically cleaves activated coagulation factors Va and VIIIa, thereby preventing the generation of activated coagulation factors Xa and thrombin. Murine thrombomodulin is initially produced with an N-terminal propeptide (a.a. 1-16) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 17-517), a transmembrane segment (a.a. 518-541), and a short cytoplamic tail (a.a. 542-577).

References

Product Information
Format	Purified
Presentation	Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Thrombomodulin Antibody, clone 411-201B is an antibody against Thrombomodulin for use in Immunohistochemistry (Paraffin), Electron Microscopy, Immunoprecipitation, Western Blotting, ELISA.
Key Applications	Immunohistochemistry (Paraffin) Electron Microscopy Immunoprecipitation Western Blotting ELISA
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human kidney tissue. Electron Microscopy: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). Immunoprecipitation Analysis: A representative lot immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblast (NMLF) lysates, but not from thrombomodulin-depleted NMLF lysates by performing IP with clone 273-34A (Cat. No. MABS1245) prior to IP with clone 411-201B (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). Western Blotting Analysis: A representative lot detected thrombomodulin (P112) in the normal mouse lung fibroblast (NMLF) immunoprecipitates obtained with either clone 411-201B or clone 273-34A (Cat. No. MABS1245) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). Immunohistochemistry Analysis: Representative lots and clone 273-34A (Cat. No. MABS1245) showed similar staining pattern using both frozen and paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Rorvik, M.C., et al. (1988). J. Histochem. Cytochem. 36(7):741-749; Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). ELISA Analysis: Representative lots were used as the detection antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 273-34A (Cat. No. MABS1245) as the capture antibody and an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay employing 125I-labeled clone 411-201B without an enzyme-conjugated secondary antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450).

Biological Information
Immunogen	Normal mouse lung homogenate.
Epitope	Extracellular domain.
Clone	411-201B
Concentration	Please refer to lot specific datasheet.
Host	Rat
Isotype	IgG2aκ
Species Reactivity	Mouse Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_033404.1
Gene Symbol	Thbd
Purification Method	Protein G Purified
UniProt Number	P15306
Molecular Weight	61.87 kDa calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry in mouse lung tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Thrombomodulin in mouse lung tissue.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABS1273	04055977312461

Documentation

Anti-Thrombomodulin Antibody, clone 411-201B MSDS

职位
物料安全数据表 (MSDS)

Anti-Thrombomodulin Antibody, clone 411-201B 分析证书

标题	批号
Anti-Thrombomodulin, clone 411-201B - 3422747	3422747
Anti-Thrombomodulin, clone 411-201B - 3422825	3422825
Anti-Thrombomodulin, clone 411-201B - 3823277	3823277
Anti-Thrombomodulin, clone 411-201B -Q2639561	Q2639561

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描述

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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